Volume 31, Issue 5 (August 2020)                   Studies in Medical Sciences 2020, 31(5): 364-371 | Back to browse issues page

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Associate Professor, Department of Anatomy, Anatomy and Embryology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran (Corresponding Author) , ahmadiabbas36@yahoo.com
Abstract:   (2379 Views)
Back ground & Aims: In this experimental study, the effect of hypotaurine on in vitro fertilization and fertility of oocytes from mice with experimental polycystic ovary syndrome was designed and performed.
Materials & Method: In this experimental study, NMRI mice aged 6 to 8 weeks were used. The mice were divided into two groups, control and experimental PCOS. For induction of experimental PCOS, Estradiol Valerate (100 mg/kg, IP) was injected. Then, for ovulation, the injection was performed first with 7.5 IU PMSG in the volume of 0.1 ml and 46-48 hours later with 7.5 IU hCG in the volume of 0.1 ml. Finally, 0/1, 1 and 10 μM curcumin were added to the culture medium of oocytes of PCOS group and development in different groups were evaluated (P <0.05).
Results: Most of the arrested embryos had a high percentage of lysis and fragmentation and the arrested embryos were type I and II. The addition of hypotaurin reduced the rate of lysis and fragmentation and decreased the percentage of arrested embryos. Lesser arrested embryos were type III (P <0.05). This study showed that 1 μM hypotaurin increased fertilization percentage and 10 μM hypotaurin increased the percentage of two-cell embryos and also increased the percentage of blastocytes (P <0.05).
Conclusion: The results showed that addition of certain concentrations of hypotaurin as an antioxidant, improved and increased the fertilization rate and the percentage of two-cell embryos. It can also be helpful in increasing the percentage of blastocysts.
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Type of Study: Research | Subject: فیزیولوژی

1. Ndefo UA, Eaton A, Robbinson M. Polycystic ovary syndrome 2013; 38: 336-338. [PMCID]
2. Dumesic DA, Schramm RD, Abbott DH. Early Origins of Polycystic Ovary Syndrome (PCOS). Reprod Fertil Dev 2005; 17:349-360. [DOI:10.1071/RD04092] [PMID]
3. Teede HJ, Misso ML, Deeks AA, Moran LJ, Stuckey BGA, Wong JLA, et al. Assessment and management of polycystic ovary syndrome: summary of an evidence-based guideline. Med J Aust 2011; 195: 65. https://doi.org/10.5694/j.1326-5377.2011.tb04131.x [DOI:10.5694/mja11.10915]
4. Daniel A. Dumesic, Vasantha P, and David H. Abbott. Polycystic Ovary Syndrome and Oocyte Developmental Competence. Obstet Gynecol Surv 2008; 63(1): 39-48. [DOI:10.1097/OGX.0b013e31815e85fc] [PMID] [PMCID]
5. Leila A, Najmeh T, Mansoureh M, Fahimeh RT, and Saeedeh Z. Antioxidants and management of polycystic ovary syndrome in Iran, Jan 2015; 13(1): 1-8. [PMID] [PMCID]
6. Hansen PJ, In Vitro Production of Bovine Embryos. Department of Animal Sciences, University of Florida 2013, Version 10-16. [URL]
7. Chian RC, In-vitro maturation of immature oocytes for infertile women with PCOS. Reprod Biomed Online 2004;8(5):547-52. [DOI:10.1016/S1472-6483(10)61101-7] [PMID]
8. Bansal AK and Bilaspuri GS, Impact of oxidative stress and antioxidants an semen functions. Vet Med Int 2011; ID 686137, 7. [DOI:10.4061/2011/686137] [PMID] [PMCID]
9. Ahmadi A, And Sadrkhanlo R. Study of Antioxidant Effects of Hypotaurine on Reduction of Oxidative Stress in the Development of Embryos of Mice Experienced from in vitro fertilization. Urmia Med J 2010; 21: 377-503. [URL]
10. Carbone MC, Tatone C, Delle SD, Marci R, Caserta D, Colonna R, et al. Antioxidant enzymatic defences in human follicular characterization and age-dependent changes. Mol Hum Reprod 2003; 9: 639-43. [DOI:10.1093/molehr/gag090] [PMID]
11. Kalir A, and Henry H. Biological activity of sulfinic acid derivatives in chemistry of sulphinic acid, esters their derivatives edited by saul. Wiley, New York 1990; P. 665. [DOI:10.1002/9780470772270.ch23]
12. Horst VD, Brand A. Occurrence of hypotaurine and inositol in the reproductive tract of the ewe and its regulation by pregnenolone and progesterone. Nature 1969; 223:67-8. [DOI:10.1038/223067a0] [PMID]
13. Roe AH, Dokras A. The Diagnosis of Polycystic Ovary Syndrome in Adolescents. Rev Obstet Gynecol 2011; 4: 45-51. [PMID] [PMCID]
14. Shannon M, Wang Y. Polycystic ovary syndrome: A common but often unrecognized condition. J Midwifery Womens Health 2012; 57:221-30. [DOI:10.1111/j.1542-2011.2012.00161.x] [PMID]
15. Sushma R, Nazi B, Sumith M, and Vassudha B. Beneficial effect of Curcumin in Letrozole induced polycystic ovary syndrome, science direct 2016; 5(2) 116-22. [DOI:10.1016/j.apjr.2016.01.006]
16. Teruhiko W, Jun-ihi S, Kenkichi I, Yutaka T, Masamichi K, and Yoshihiro H. Effect of Hypotaurine on in Vitro Fertilization and Production of Term Offspring from in Vitro-Fertilized Ova of the Japanese Field Vole, Microtus Montebelli. Biology of Reproduction 1996; 54(3): 625-30. [DOI:10.1095/biolreprod54.3.625] [PMID]
17. Meizel S, Lui CW, Working PK, Mrsny RJ. Taurine and hypotaurine: their effects on motility, capacitation and the acrosome reaction of hamster spermatozoa in vitro and their presence in spermatozoa and reproductive tract fluids of several mammals. Dev Growth Differ 1980; 22:483-94. [DOI:10.1111/j.1440-169X.1980.00483.x]
18. Okezie IA, Barry H, Brigid MH. and John B. The antioxidant action of taurine, hypotaurine and their metabolic precursors. Biochem 1988; 256, 251-5. [DOI:10.1042/bj2560251] [PMID] [PMCID]
19. Mario F, Donatella A, Emanuela O, Alberto B, and Laura P. Oxidation of hypotaurine and cysteine sulphinic acid by peroxynitrite. Biochem 2005; 389. 233-240. [DOI:10.1042/BJ20041696] [PMID] [PMCID]
20. Mario FFG, and Laura P. The protective effect of hypotaurine and cysteine sulphinic acid on peroxynitrite-mediated oxidative reactions. Free Radical Research 2008; 42(4): 320-30. [DOI:10.1080/10715760801999727] [PMID]
21. Donelly ET, Mclure N, and Lewis SE. Glutathione and hypotaurine in vitro: effects on human sperm motility, DNA integrity and production of reactive oxygen species. Mutagenesis 2000; 15(1):61-8. [DOI:10.1093/mutage/15.1.61] [PMID]

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