2024-03-29T19:09:48+03:30 http://umj.umsu.ac.ir/browse.php?mag_id=157&slc_lang=fa&sid=1
157-5552 2024-03-29 10.1002
Studies in Medical Sciences Studies in Medical Sciences 2717-008X 2717-008X 10.61186/umj 2021 32 5 CYTOTOXIC EFFECTS OF ARTEMISIA ABSINTHIUM EXTRACT ON A2780 CELL LINE (OVARIAN CANCER) AND ALTERATION OF APOPTOTIC GENES EXPRESSION LEVELS Saeedeh Khang nikfarjam saeedehnikfarjam@gmail.com Javad Baharara baharara78@gmail.com Khadijeh Nejad shahrokhabady Shahrokhabady@yahoo.com Background & Aims: Ovarian cancer is the third most common cancer in women. Artemisia is one of the most commonly used medicinal plants, and Artemisia absinthium is one of the important species of this genus. The aim of this study was to evaluate the effect of cytotoxicity and the ability to induce apoptosis methanolic extract of A. absinthium in A2780 cell line (human ovarian cancer). Materials & Methods: In this experimental study, methanolic extract of A. absinthium was prepared. MTT assay was used to evaluate the cytotoxic effects of A. absinthium extract on A2780 cancer cell proliferation and DAPI staining and flow cytometry analysis were used to evaluate the ability to induce apoptosis in treated cells with A. absinthium extract. Changes in "BAX, Caspase 3,9, p53" genes expression were evaluated by Real-time PCR. Quantitative data were analyzed by one-way ANOVA at a significant level of p<0.05. Results: The MTT assay results showed that methanolic extract of A. absinthium, inhibits proliferation of ovarian cancer A2780 cells in a concentration-dependent manner (p<0.001***). Morphological observation with DAPI staining and flow cytometry analysis results showed an increased percentage of apoptotic cells. The real-time PCR results showed an increase in the expression of apoptotic genes "BAX, Caspase 3,9, p53" in treatment groups p <0.01**; p<0.001***. Conclusion: The results of this study showed that the methanolic extract of A. absinthium inhibits the proliferation of A2780 cancer cells and induces apoptosis in these cells. Ovarian cancer Apoptosis Artemisia absinthium 2021 8 01 317 328 http://umj.umsu.ac.ir/article-1-5552-en.pdf
157-5380 2024-03-29 10.1002
Studies in Medical Sciences Studies in Medical Sciences 2717-008X 2717-008X 10.61186/umj 2021 32 5 TUBERCULOUS OSTEOMYELITIS IN THUMB: A RARE CASE Keykhosro Mardanpour kmardanpour@yahoo.com Mahtab Rahbar rahbarahbar@ Although tuberculous osteomyelitis is rare in the general population, it should still be considered as a threat to human health. A 52-year-old woman acutely develops a progressively painful swelling on the tip of her right thumb.  On laboratory examination, there is no abnormality except for a slight increase in acute phase inflammatory tests (ESR and CRP). A right-hand x-ray showed an expansile lytic lesion. Microscopic examination revealed tuberculosis granuloma. Anti-tuberculous chemotherapy started for two months with a regimen composed of isoniazid, rifampicin, pyrazinamide, and ethambutol and then followed by a ten month regimen consisting of isoniazid and rifampicin. Bone tuberculosis should always be considered by physicians, as early diagnosis and treatment will prevent additional serious complications of the disease. Extrapulmonary tuberculosis tuberculous osteomyelitis thumb 2021 8 01 329 334 http://umj.umsu.ac.ir/article-1-5380-en.pdf
157-5499 2024-03-29 10.1002
Studies in Medical Sciences Studies in Medical Sciences 2717-008X 2717-008X 10.61186/umj 2021 32 5 COMPARISON OF THE RELATIONSHIP BETWEEN PROCALCITONIN AND CRP WITH PROGNOSIS OF FEVER AND NEUTROPENIA IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA Farid Ghazizadeh gazizadef@gmail.com Mehran Noroozi mehranxnoroozi@gmail.com Mahsa Shekari mahsashekari63@gmai.com Background & Aims: The present study aimed to compare the relationship between Procalcitonin and CRP with a prognosis of fever and neutropenia in children with acute lymphoblastic leukemia. Materials & Methods: In this descriptive-analytical study, patients with fever and neutropenia who underwent chemotherapy were included. Duration of fever, length of hospital stay, mortality rate, Procalcitonin and CRP levels, and other patient information were included in the checklist and analyzed. Results: In this study, 31 patients with fever and neutropenia were enrolled. The results showed that subjects with high procalcitonin levels had a longer duration of fever and hospitalization than those with lower levels of Procalcitonin. A statistically significant level was also obtained. The results of our study showed that there is a significant relationship between the level of Procalcitonin and sepsis (p = 0.001); however, the CRP level was not significantly correlated (p = 0.372). Conclusion: Procalcitonin might be an adjunctive biomarker in identifying severity of disease, duration of antimicrobial therapy and choosing the right antibiotic for cancer patients with fever and neutropenia. Procalcitonin-guided algorithm may limit the duration of antibiotics, reduce adverse events, and prevent the emergence of antimicrobial resistance.   Fever and Neutropenia Procalcitonin CRP 2021 8 01 335 341 http://umj.umsu.ac.ir/article-1-5499-en.pdf
157-5500 2024-03-29 10.1002
Studies in Medical Sciences Studies in Medical Sciences 2717-008X 2717-008X 10.61186/umj 2021 32 5 COMPARISON OF SENSITIVITY AND SPECIFICITY OF LAL/TOC ANIMAL PYROGEN (RABBIT) TEST WITH DIFFERENT DILUTIONS OF E.COLI FOR THE ASSESSMENT OF QUALITY OF WFI IN PHARMACEUTICS Parisa Bigdeli Ezzat Nourizade Background & Aims: Today, parenteral products occupy a particular place in the treatment of diseases and human health. Consequently, the presence of any microbial contamination in the water used in the drug production process may have adverse effects on the health of individuals and society. Since parenteral sera are in direct contact with the human body and blood, they must be free from pyrogens. The purpose of this study was to evaluate WFI water quality in the pharmaceuticals industry by LAL test methods and TOC test and rabbit pyrogen test with different dilutions of E. coli. Materials & Methods: In the present study, the sensitivity and specificity of LOL, TOC, and rabbit pyrogen tests with different dilutions of E. coli were evaluated to assess the water quality of WFI in the pharmaceuticals industry. LAL kits, EMB, TOC set, Spectrometer, Colony counter and Rabbits and… wad used in this study Results: None of the diluted solutions of E. coli were appropriate for medicinal water. The LAL test also gave more accurate results than the rabbit test, although it still appears that the LAL test is not entirely accurate. Conclusion: The results showed that all methods have advantages and disadvantages compared to one another, but the use of the TOC device in the thinnest samples demonstrates more acceptable results. LAL TOC Pyrogen E. coli WFI 2021 8 01 342 353 http://umj.umsu.ac.ir/article-1-5500-en.pdf
157-5292 2024-03-29 10.1002
Studies in Medical Sciences Studies in Medical Sciences 2717-008X 2717-008X 10.61186/umj 2021 32 5 EFFECTIVENESS OF COMBINATION OF TRANSCRANIAL DIRECT CURRENT STIMULATION AND MINDFULNESS-BASED GROUP COGNITIVE THERAPY ON DEPRESSION REDUCTION IN NONCLINICAL POPULATIONS Akbar Mahdiloo stu.mahdiloo@iaut.ac.ir Naeimeh Moheb moheb@iaut.ac.ir seyed Mahmoud Tabatabaei smt@iaut.ac.ir Marzieh Alivandi Vafa m.alivand@iaut.ac.ir Background & Aims: Depression is the most common mental disorder in the world. Treatment with transcranial direct current stimulation is one of the new treatments, and mindfulness-based group cognitive therapy is one of the effective treatments for depression. The purpose of this study was to evaluate the efficacy of the combination of these two therapies in reducing depression. Materials & Methods: This was a quasi-experimental design with pre-test, post-test with control group. Subjects were 75 students with depressive symptoms who were selected by purposeful method based on the Beck Depression Inventory (score above 15) and then they were randomly divided into 5 groups (Including 3 experimental groups, sham group and control group) and re-evaluated in post-test using the Beck Depression Inventory. The data were analyzed by analysis of covariance using SPSS 21 software. Results: The results showed that all experimental groups had a significant decrease in post-test depression scores (p <0.05) while in the sham and control groups there was no significant difference in post-test (p> 0.05). There was also a significant decrease in depression scores in the combination of transcranial direct current stimulation and mindfulness-based group cognitive therapy compared to the other groups. Conclusion: Combining transcranial direct current stimulation and mindfulness-based group cognitive therapy is effective in reducing depression in the nonclinical population. Depression Mindfulness-based group cognitive therapy Transcranial direct current stimulation 2021 8 01 354 364 http://umj.umsu.ac.ir/article-1-5292-en.pdf
157-5540 2024-03-29 10.1002
Studies in Medical Sciences Studies in Medical Sciences 2717-008X 2717-008X 10.61186/umj 2021 32 5 INVESTIGATION OF THE EXPRESSION LEVEL OF MIR-195 AS A POTENTIAL BIOMARKER IN THE PLASMA OF GASTRIC CANCER PATIENTS IN GUILAN PROVINCE Fatemeh Asadi Rahmani n.asadirahmani@gmail.com Najmeh Ranji najmehranji@gmail.com Hamid Saeidi Saedi hamidsaedi53@yahoo.com Background & Aims: Gastric cancer is one of the five most common cancers in Iran, the sixth most common cancer in the world, and the second cause of cancer-related death worldwide. Failure to diagnose gastric cancer in early stages suggests the need to find potential biomarkers. This study aimed to evaluate the miR-195 expression as a potential biomarker in the plasma of gastric cancer patients in Guilan province. Materials & Methods: In this case-control study, the Q-RT-PCR method was used to evaluate the expression level of miR-195 in the plasma of 25 patients with gastric cancer and 25 normal individuals. The relationship between the expression level of miR-195 and clinicopathologic features was evaluated using the ANOVA test. The receiver operating characteristic (ROC) and area under the curve (AUC) were used to evaluate the miR-195 as a potential biomarker to discriminate cancerous from non-cancerous states of the samples. Results: Our analysis showed that miR-195 was significantly downregulated in gastric cancer patients compared to the normal individuals (p˂0.05). The AUC value of ROC analysis was 0.7325±0.008 (p=0.0119) with the sensitivity of 51.7% and specificity of 71.8%. Conclusion: Downregulation of miR-195 in gastric cancer patients and ROC analysis suggested that miR-195 can be introduced as a potential biomarker in the diagnosis of gastric cancer in Guilan province. Biomarker Gastric cancer miR-195 Plasma ROC analysis 2021 8 01 365 375 http://umj.umsu.ac.ir/article-1-5540-en.pdf
157-5425 2024-03-29 10.1002
Studies in Medical Sciences Studies in Medical Sciences 2717-008X 2717-008X 10.61186/umj 2021 32 5 EFFECTS OF ANIMAL OILS COMPARED TO VEGETABLE OILS ON HISTOPATHOLOGY AND LIVER ENZYMES: INTERVENTIONAL AND EXPERIMENTAL STUDY Behrooz Yahyaei behroozyahyaei@yahoo.com Jafar Tahmasebi dr.tahmasebi.j@gmail.com Moein Mehri moeinmehri2727@gmail.com Background & Aims: This study aimed to investigate the effects caused by butter and fat animal oils in camparison to rapeseed and sesame oils on liver histopathology and liver enzymes in rats. Materials & Methods: This experimental study was performed on 36 adult male and female rats (20±200 g). The studied groups were fed by two levels of 10 and 20% and including sesame oil, canola oil, tail and animal butter; the control group was fed according to the standard without using the above oils. At the end of 6 weeks of weight change, Alkaline Phosphatase (ALP), Alanine Transaminase (ALT) and Aspartate Aminotransferase (AST) levels were measured. Results: Diet containing 10% tail (202.5 g), and 20% butter and sesame (196.75 g) caused weight gain, which compared to butter and sesame, sesame caused more weight gain (p˃0.05). Butter and sesame oil at both levels of 10% and 20% in the diet of male and female rats reduced the amount of ALP, which in comparison to butter and sesame can be said to lead to a further reduction in ALP (226.75). Rapeseed and tail oil increased ALP levels by 10% and 20% at both levels. Compared to rapeseed and tail oil, it can be concluded that rapeseed oil increased ALP (559 mg). A higher increase in ALT was observed in diets containing 10% of tail (114.75 mg) and in 20% of rapeseed (139.5 mg). Vegetable oils of sesame and rapeseed and butter and tail oils in the diet at the level of 10 and 20% led to an increase in AST, which at the level of 10% butter (211.25 mg) and at the level of 20% sesame caused a significant increase in AST (233.5 mg). Conclusion: Animal oils cause weight gain in both fat levels while vegetable oils cause weight loss. Vegetable and animal oils at both fat levels increase AST, and vegetable and tail oils at both fat levels increase ALT. Butter and sesame oils at both levels decrease and rapeseed and tail oils at both levels increase ALP. Histology also showed the highest changes in the level of 20% of rapeseed and sesame oils and then butter, respectively. Liver enzyme Rat Animal oil Vegetable oil 2021 8 01 376 387 http://umj.umsu.ac.ir/article-1-5425-en.pdf
157-5557 2024-03-29 10.1002
Studies in Medical Sciences Studies in Medical Sciences 2717-008X 2717-008X 10.61186/umj 2021 32 5 The Effectiveness of Existential Therapy on Death Anxiety and Meaning of Life in Recovered Patients of Covid-19 Sara Alizadeh s.alizadeh@iauardabil.ac.ir Somayyeh Taklavi staklavi@gmail.com Majid Mahmoud Alilou M-alilou@tabrizu.ac.ir Haedeh Feizipour H.feizipour@gmail.com Background & Aims: Due to the high physiological and psychological damage of COVID-19 patients after discharge from the hospital and the need for interventional approaches, the present study was conducted to evaluate the effectiveness of existential therapy on death anxiety and the meaning of life in recovered COVID-19 patients. Materials & Methods: The research was a quasi-experimental design with pre-test and post-test and control group. The statistical population in this study included all recovered patients of COVID-19 in 2020 in Urmia, Iran. To select the sample, the patients' files were checked in the counseling center of Taleghani Hospital. After that, 30 of the recovered patients that had the inclusion criteria were randomly assigned to either experimental or control groups, each group consisting of 15 individuals. The experimental group received ten sessions of online existential therapy whereas the control group received no intervention. For the collection of data, the Meaning of Life (MLQ) and Death Anxiety Questionnaire (DAQ) were used. The collected data were analyzed using single-factor analysis of covariance (ANCOVA). Results: The results of the comparison of the post-tests of the groups indicated that scores of death anxiety (p<0.01) and meaning of life (p<0.01) of experimental and control groups differed significantly. The analysis of data showed that existential therapy reduced death anxiety and increased the meaning of life among recovered patients of COVID-19. Conclusion: Existential therapy is an effective approach in dealing with critical situations. The results of the present study can be used to improve the perplexities related to death and the meaning of life during the COVID-19 pandemic. Existential Therapy Meaning of Life Death Anxiety Recovered Patients of COVID-19 2021 8 01 388 398 http://umj.umsu.ac.ir/article-1-5557-en.pdf