Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
7
2018
10
1
DELETION AND POINT MUTATION ANALYSIS OF THE SMN1 GENE IN PATIENTS WITH SPINAL MUSCULAR ATROPHY (SMA) IN WEST AZERBAIJAN PROVINCE OF IRAN
474
480
FA
MORTEZA
BAGHERI
Urmia University of Medical Sciences
ISA
ABDIRAD
Urmia University of Medical Sciences
AHAD
GHAZAVI
Urmia University of Medical Sciences
Background & Aims: Spinal muscular atrophy (SMA) is a common disorder with autosomal recessive inheritance pattern. The frequency of carriers of this disease is one in forty to one in sixty. SMA occurs in 98% of cases due to the homozygous deletion of SMN1 exons 7 and 8. The purpose of this study was to evaluating the deletion and point mutations of the SMN1 gene in patients with SMA in west Azerbaijan province of Iran.
Materials & Methods: A total of 50 patients with SMA were referred to the Genetic Department after clinical diagnosis for molecular evaluation and genetic counseling. Genomic DNA was extracted from blood samples. The exclusion rate of exons 7 and 8 in the neuronal survival gene 1 was determined by using the PCR-RFLP. Results: Deletion of exons 7 and 8 were observed in 98% of the studied cases (49 out of 50 cases). In one patient, the sequencing of exon 5 showed homozygote mutation c.549 del C (p.Lys184ser fs 29) (point mutation).
Conclusion: The evaluation of the presence or absence of exons 7 and 8 of the SMN1 gene, as well as point mutations in SMN1 gene in patients suspected of musculoskeletal atrophy, is effective in confirming the clinical diagnosis and subsequent genetic counseling.
Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
7
2018
10
1
The Effect of 12 Weeks Moderate Intensity Aerobic training on Serum Leptin, GH / IGF-1 in Adult and Immature Inactive Girl Students
481
493
EN
Javad
Tolouei Azar
Faculty of Sport Sciences, Urmia University of Medical Sciences
Asghar
Tofighi
Faculty of Sport Sciences, Urmia University, Urmia, Iran
narmin
motab saei
Faculty of Sport Sciences, Urmia University, Urmia, Iran
Background & Aims: puberty is a process in which physical and physiological changes lead to changes in the child's body as an adult with reproductive capacity. The purpose of the present study was to investigate the effect of 12 weeks of moderate intensity aerobic training on serum leptin, growth hormone / Insulin-Like Growth Factor-1, in Mature and immature Inactive Girl Students.
Materials & Methods: 40 non-athlete girl students aged 10 to 15 years were matched according to Tanner scale at stages 1, 2 and 3, 4 of puberty and each group was divided into two experimental and control groups. Experimental groups (n = 20) performed 3 sessions per week for 12 weeks, each session performed aerobic training with 45-65% of maximal heart rate for 45 minutes, control groups (20 subjects) in no intervention they did not attend. Blood samples were measured before and after the exercise training. For data analysis, Kolmogorov-Smirnov test (k-s), Leven test, ANCOVA, and P-value <0.05 were used for data analysis.
Results: In experimental groups compared with control groups, after 12 weeks of aerobic training, a significant decrease in leptin and Insulin-Like Growth Factor-1 (P <0.001) and a significant increase in growth hormone (P <0.001) was observed.
Conclusion: See also, moderate-intensity aerobic training appears to have resulted in significant changes in the hormonal indices that are effective in puberty, which indicates the desired effect of type, intensity, duration of activity in girl students inactive.
Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
7
2018
10
1
Conditioned medium derived from mesenchymal Stem cells regenerates’ defected articular cartilage
494
501
EN
Masoumeh
Zirakjavanmard
Department of Anatomy, Urmia University of Medical Sciences
mojtaba
karimipour
Department of Anatomy, Urmia University of Medical Sciences
Fatemeh
. Atabaki Pasdar
Department of Anatomy, Urmia University of Medical Sciences
Background & Aims: One of cell- based technical issues associated with cartilage repair assay is delivering cells to the site of the parts where damage is created. Mesenchymal stem cells (MSCs) with their chondrogenic potential are ideal candidates for cartilage regeneration. High expression of cartilage hypertrophy markers by MSCs would result in apoptosis and ossification. This investigation is attempted to find out if paracrine factors secreted from MSCs can regenerate damaged articular in a rat model.
Materials & Methods: Osteoarthritis (OA) was induced in eighteen adult rats by intra- articular (IA) injection of monosodium iodoacetate (MIA) into right knee. Then the animals were randomly divided into three groups (n=6). Control group received IA injection of vehicle; MSCs group received a single IA injection of MSCs (2.5×105/50µL DMEM) and conditioned medium(CM) group received a single IA injection of CM, meanwhile six left intact knee joints were taken as the normal group. After four weeks, the animals were scarified by overdose injection of ketamine and then, the distal end of femur removed and fixed in formalin. After decalcification using formic acid, histological slides obtained. In this study number of chondrocytes and morphology of articular surface were evaluated.
Results: Four weeks after treatment, gross and microscopic evidence of articular cartilage of distal end of femur cartilage demonstrated the significant (p<0.05) healing of OA joints in group treated with MSCs. Also, in CM group significant increase in number of chondrocyte (p<0.05) and decrease of degenerated holes width was observed compared to the control group.
Conclusion: Given the deficiencies of cell-therapy in OA, a potential for repair of degenerated articular cartilage is thought possible to be represented by cell free CM.
Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
7
2018
10
1
Role of PA3574 (nalD) gene in development of ciprofloxacin resistance in Pseudomonas aeruginosa isolates
502
510
EN
Saeid
Rahbar Takrami
rasht branch , islamic azad university
najmeh
ranji
rasht branch , islamic azad university
Background & Aims: Pseudomonas aeruginosa is a gram-negative opportunistic pathogen and one of the mortality causes of nosocomial infections. One of the drug resistance mechanisms in P.aeruginosa is mutation in negative regulator genes of mexAB-oprM efflux pump system such as nalD. The aim of this study was to investigate the role of nalD mutations in P. aeruginosa isolates of Guilan province in ciprofloxacin resistant development.
Materials & Methods: In this study, 45 P. aeruginosa isolates were obtained from different clinical samples of Rasht and Lahijan hospitals and laboratories between 2014 to 2016 and identified by biochemical tests. Ciprofloxacin resistance and susceptibility of the strains was determined by disc difusion and MIC methods. PCR-sequencing was performed to assess nalD gene mutations in ciprofloxacin resistant strains.
Results: Seventeen from 45 isolates of P.aeruginosa were ciprofloxacin resistant (MIC≥1024 µg/ml). PCR-sequencing analysis showed that one resistant isolate had one- C nucleotide deletion in codon 193 (p.Leu193CysfsX, in gene level: c.577delC)) (c.577delC) and three resistant isolates had L153Q missense mutations in nalD gene.
Conclusion: It appears that mutations of nalD gene lead to overexpression of mexAB-oprM and subsequently ciprofloxacin resistance in P.aeruginosa isolates in Guilan province.
Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
7
2018
10
1
Effects of 17-β estradiol on neurogenesis in the hippocampus of ovariectomized mice
511
521
EN
Maryam
Azari
School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran
Mohammad Taghi
Ghorbanian
School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran
Background & Aims: Adult neurogenesis occurs in the two main areas of the brain of most mammalian species in; sub ventricular zone, and the dentate gyrus of the hippocampus. Many factors such as 17-β estradiol affect neurogenesis in the hippocampus. The aim of this study was to investigate the effect of exogenous 17-β estradiol on neurogenesis in the ovariectomized (OVX) mice.
Materials & Methods: NMRI mice were divided into five experimental groups: 1- Sham, 2- Control, 3- Treated with single dose of 17-β estradiol two weeks after ovariectomy and euthanized 24 hours later, 4- Treated with single dose of 17-β estradiol two weeks after OVX and euthanized 48 hours later, 5- Treated with single dose of Sesame Oil (vehicle) 2 weeks after OVX and euthanized after 24 hours. Animals were perfused transcardially with paraformaldehyde. Brains were removed and its sections for cresyl fast violet staining and Glial fibrillary acidic protein (GFAP) and BrdU immunohistochemistry were prepared. Cells were counted and investigated using light and fluorescent microscopy.
Results: Neuronal density and Proliferation of hippocampal progenitor cells in the CA1 region of 17-β estradiol -treated mice were significantly increased up to 24 hours. The density of astrocytes in different region of the hippocampus was significantly increased after treatment by 17-β estradiol.
Conclusion: Cell shape and density of hippocampal CA1 neurons are influenced by 17-β estradiol. In addition, density and morphology of glial cells, especially astrocytes in different regions of the hippocampus are affected by 17-β estradiol. Thus, 17-β estradiol can improve neurogenesis in the CA1 region of the hippocampus.
Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
7
2018
10
1
Automated differentiation of benign and malignant liver tumors by Ultrasound Images
522
529
EN
Milad
Zeinali Kermani
Urmia University of Medical Sciences, Urmia, Iran
Akbar
Garbali
Urmia University of Medical Sciences, Urmia, Iran
Background & Aims: Early detection and reliable differentiation of benign and malignant liver tumors could lead to improved cure rate and costs. Ultrasound image (US) is a convenient medical imaging method for interpreting liver tumors. Visual inspection of ultrasound images sometimes is combined with error and needs biopsy to confirm whether a tumor would be benign or malignant. The aim of this study is to explore the potential of computerize texture analysis methods for classifying benign and malignant liver tumors in US imaging.
Methods and materials: The US image database comprised 38 liver patients (25 malignant and 13 benign).Up to 270 texture features parameters as descriptors computed for each selected region of interest (ROIs) under default normalization scheme. Two feature reduction methods: Fisher and POE+ACC algorithms are applied to find the most effective features to differentiate benign from malignant liver. Obtained features parameters under two standardization states: standard (S) and nonstandard (NS) were used for texture analysis with PCA and LDA. Finally, Receiver Operating Characteristic (ROC) curve analysis was used via calculating sensitivity, specificity accuracy and Az value (area under the ROC curve) to examine the discrimination performance of applied texture analysis methods.
Results: The very excellent performance for discrimination between benign and malignant liver tumors was recorded for LDA with sensitivity of 98.7%, specificity of 100% and Az value of 1. Also, for PCA discrimination results has sensitivity of 98.6%, specificity of 100% and Az value of 0.99.
Conclusion: Our results indicates that texture analysis of the liver US images has potential to increase confidence of radiologist in classification of benign from malignant liver tumors.
Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
7
2018
10
1
ASSOCIATION STUDY OF MIR-146A RS2910164 AND RS57095329 POLYMORPHISMS WITH RISK OF BEHCET’S DISEASE
530
538
EN
Shohreh
Zare-Karizi
Varamin Pishva Branch, Islamic Azad University, Varamin Pishva,
Ziba
Kohansal Vajargah
Varamin Pishva Branch, Islamic Azad University, Varamin Pishva,
Reza
Mirfakhraie
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Background & Aims: Behcet's disease (BD) is an autoimmune disease that usually occurs with oral, genital and ocular ulcers. The cause of this disease is unknown and environmental, genetic and immunological factors contribute to its development. In the present study, the relation between rs2910164 and rs57095329 polymorphisms of miR146a gene with the potential for BD was investigated in Iranian population.
Materials & Methods: In this case-control study, 100 patients with Behcet's disease and 100 Normal with no history of disease were studied as control. The polymorphism rs2910164 was determined by PCR-RFLP method using the MnlI enzyme, and the polymorphism rs57095329 was determined by the ARMS PCR method for genotype. The data were analyzed using SPSS version 22.
Results: The prevalence of GG genotype in rs2910164 polymorphism showed no significant difference between patient and control groups (p=0.156). The Frequency of the genotype AA was also not significantly different in the rs57095329 polymorphism in both patient and control groups (p=0.814).
Conclusion: In this study, there was no significant relationship between rs2910164 and rs57095329 polymorphisms and the potential for BD. Research on a greater number of patients can be effective.
Urmia University of Medical Sciences
Studies in Medical Sciences
2717-008X
29
7
2018
10
1
Investigation of the effect of experimental polycystic ovarian syndrome induced by stradiolvalerate on oocyte quality and in vitro fertilization potential and evaluation of vitamin E supplementation to emryo culture in mouse model
539
549
EN
morvarid
Poshdar
Urmia University, veterinary college, department of Basic Science
abbas
Ahmadi
Department of Veterinary Medicine, Urmia, Iran
mahdi
Imani
Department of Veterinary Medicine, Urmia, Iran
Background & Aims: One of the most common causes of infertility in women is ovarian causes, especially polycystic ovary syndrome (PCOS). However, in patient with PCOS, the number of oocytes taken increased during the IVF, but these oocytes have often low quality, which ultimately leads to reduce fertilization potential. The aim of this research was investigation of PCOS on oocytes quality and potential of IVF and and evaluation of the effect of vitamin E supplementation to embryonic culture medium, as well.
Materials & Methods: In this experimental syudy100 female mice were divided into 2 groups: PCOS and control. To create an experimental PCOS stradiolvalerate injected itraperitonealy (100mg/kg). After 8 weeks IVF process was performed and the quality of oocytes, fertilization rate and embryo development process were evaluated. Finally, the effect of vitamin E with doses of 100, 200, 400 micromol, (p<0.05) were investigated in embryo culture.
Results: Comparison of results showed that experimental PCOS group showed a significant decreasein oocyte quality and fertilization, dualcell, blastocyst and significant increase in lysis and fragmentation in stopped embryos, on the other hand lysis and fragmentation, reduction of percentage and type of stopped embryos in presence of 100 and 200 micromole vitamin E concentrations were significantly in compare with PCOS group (p<0.05).
Discussion & Conclusion: Finally it can be concluded that experimental PCOS decreases the quality of oocytes and IVF and the addition of vitamin E to fetal culture medium as antioxidant can improve fertility.