Studies in Medical Sciences
مجله مطالعات علوم پزشکی
Studies in Medical Sciences
Medical Sciences
http://umj.umsu.ac.ir
37
journal37
2717-008X
2717-008X
10.61186/umj
fa
jalali
1400
12
1
gregorian
2022
3
1
32
11
online
1
fulltext
en
تاثیر نانو حامل های لیپوزومی حاوی ادوجونت بر رفتار وابسته به دوز داروی پکلی تاکسل بر رویرده سلولی MDA-MB468سرطان پستان
Liposomal nanoparticles reduce dose-dependent behavior of paclitaxel against MDA-MB 468 breast cancer
اونکولوژی
Oncology
پژوهشي(توصیفی- تحلیلی)
Research
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">پیش زمینه </span></span></span></b><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">و هدف</span></span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">نانوذرات مبتنی بر انتقال دارو به عنوان یک نوآوری گسترده در پروتکل های درمان سرطان </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">ایجاد شده</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> است. در مطالعه حاضر، نقش کامپفرول</span></span></span> <span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">(KAE)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">بارگیری شده در حامل های لیپوزومی برای افزایش سمیت سلولی ، اثربخشی و آپوپتوز وابسته به پکلی تاکسل در سلولهای سرطانی پستان </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">MDA-MB 468</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> بررسی گردید.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">مواد و روش ها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> توزیع اندازه ذرات ، میکروسکوپ الکترونی روبشی (</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">SEM</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">) ، پتانسیل زتا و برداشت سلولی برای بهینه سازی و مشخص کردن لیپوزوم های بارگذاری شده با کامپفرول مورد استفاده قرار گرفت. از </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">آزمون</span></span></span> <span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">MTT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> برای ارزیابی تکثیر سلولی سلول های سرطانی استفاده شد. طبقه بندی آپوپتوز زودرس و دیررس و الگوهای بیان ژن آنها به ترتیب با رنگ آمیزی </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Annexin V/PI</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Real-time PCR</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> ارزیابی شد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">یافته ها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> تصاویر </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">SEM</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> اندازه ذرات </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">پراکنده</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> در ابعاد 3 </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%">±</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">80 نانومتر کامپفرول فرموله شده در لیپوزوم ها را ارائه داد. مقادیر </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">IC<sub>50</sub></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> برای </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">KAE</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> و پکلی تاکسل به ترتیب</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> 0.52 </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">±</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">44</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">میکرومولار و 36</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">0 </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%">±</span></span> <span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">1.75</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> نانومولار تعیین شد. تکثیر سلولی به طور متوسط از %8/26 </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%">±</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> 56 تا %9/3 </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%">±</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> 44 </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">(05/0> </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">p</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">) بعد از تیمار با </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">لیپوزوم های بارگیری شده با </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">KAE</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> گزارش گردید.</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">تجویز همزمان نانوذرات حاوی </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">KAE</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> و پکلی تاکسل در سلول های سرطانی درصد آپوپتوز را به میزان قابل توجهی تشدید کرد (05</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">0> </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">).</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> <b>نتیجه گیری:</b> نتایج ما پیشنهاد کرد که </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">KAE</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> بارگیری شده در لیپوزوم</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">s</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> به عنوان یک اجوانت ضد سرطان تکنیک قدرتمندی بوده که ممکن است سیستم دارورسانی مفید برای افزایش تأثیر عوامل شیمی درمانی بر سلول های سرطانی پستان باشد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:18.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Background & Aims:</span></i></b><b> </b><span style="font-size:9.0pt">Owing to its anti-cancer and anti-oxidant properties, Kaempferol (KAE) has become an ideal candidate to be more welcome into clinical practice. However, Due to its low water solubility and bioavailability, we aimed to design and address a new liposomal formulation with KAE and evaluate its anti-cancer activity against MDA-MB 468 breast cancer cells.</span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:18.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Materials & Methods</span></i></b><i><span style="font-size:9.0pt">: </span></i><span style="font-size:9.0pt">To characterize the physicochemical features, pharmaceutical parameters such as nanoparticle size, morphology of particles under scanning electron microscopy (SEM), and zeta potential were measured. The optimum liposomal formulation along with paclitaxel was incubated to investigate their biological activity against breast cancer cells. Furthermore, molecular mechanisms related to program cell death (apoptosis) and their gene expression were measured by flowcytometric and real-time PCR, respectively. </span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:18.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-size:9.0pt"> <b><i>Results</i></b><i>: </i>SEM images showed narrow distributed and scattered particles with the size of 80.3 nm (KAE) formulated in liposomes. IC<sub>50</sub> values for KAE and paclitaxel were determined to be as 44 ± 0.52 μM and 1.75 ± 0.36 nM, respectively. Cell proliferation averaged from 44 ± 3.9% to 56 ± 26.8% (p <0.05) after treatment with KAE-loaded liposomes. Co-administration of nanoparticles containing KAE and <a name="_Hlk82696780">paclitaxel</a> in cancer cells significantly increased the percentage of apoptosis (P <0.05). </span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:18.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Conclusion</span></i></b><i><span style="font-size:9.0pt">:</span></i><span style="font-size:9.0pt"> Taking our data into consideration, we suggest that insertion of KAE into liposomal carriers not only improved the bioavailability of this flavonoid but also surged the anti-cancer efficacy of paclitaxel.</span></span></span></span>
آپوپتوز, سرطان پستان, نانوذرات لیپوزوم, کامپفرول
Apoptosis, Breast Cancer, Kaempferol, Liposome, Paclitaxel
847
856
http://umj.umsu.ac.ir/browse.php?a_code=A-10-5130-1&slc_lang=en&sid=1
Toofan
Aghazadeh
توفان
آقازاده
Toofanaghazade@gmail.com
3700319475328460030750
3700319475328460030750
No
Student, Department of Biochemistry, Faculty of Life Sciences, Tehran North Branch, Islamic Azad University, Tehran, Iran
دانشجوی دکتری بیوشیمی، دانشکده علوم زیستی، شعبه تهران شمال ، دانشگاه آزاد اسلامی، تهران، ایران
Nuredin
Bakhtiari
نورالدین
بختیاری
Nuredin1480@gmail.com
3700319475328460030751
3700319475328460030751
No
Assistant Professor, Department of Biochemistry, Faculty of Life Sciences, Tehran North Branch, Islamic Azad University, Tehran, Iran
استادیار گروه بیوشیمی، دانشکده علوم زیستی، شعبه تهران شمال ، دانشگاه آزاد اسلامی، تهران، ایران
Isa
Abdi Rad
عیسی
عبدی راد
Isaabdirad@yahoo.com
3700319475328460030752
3700319475328460030752
No
Professor, Cellular and Molecular Research Center, Cellular and Molecular medicine Institute, Urmia University of Medical Sciences, Urmia, Iran
مرکز تحقیقات سلولی و مولکولی، انستیتو پزشکی مولکولی و سلولی، دانشگاه علوم پزشکی ارومیه، ارومیه، ایران
Fatemeh
Ramezani
فاطمه
رمضانی
Framezani82@gmail.com
3700319475328460030753
3700319475328460030753
Yes
Assistant Professor, Department of Molecular Medicine, School of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran (Corresponding Author)
دپارتمان پزشکی مولکولی، دانشکده علوم نوین پزشکی، دانشگاه علوم پزشکی تبریز، تبریز، ایران