en Evaluation of proliferation and survival of spleen immune cells treated by Deacetylchitin nanoparticles on breast cancer mouse model فیزیولوژی فیزیولوژی پژوهشي(توصیفی- تحلیلی) Research <p style="margin-left:0cm;text-align:justify;text-indent:-.1pt;line-height:18.0pt;"><strong><em><span style="font-size:9.0pt;">Background &&nbsp;Aims</span></em></strong><span style="font-size:9.0pt;">: Breast cancer is the most common carcinoma in women and one of the main causes of death in developed and developing countries. Today, compounds with immunolodulator properties can be replaced with routine drugs. One of them is Deacetylchitin. This study aimed to evaluate proliferation and survival of spleen immune cells treated by Deacetylchitin nanoparticles on breast cancer mouse model.</span></p> <p style="margin-left:0cm;text-align:justify;text-indent:-.1pt;line-height:18.0pt;"><strong><em><span style="font-size:9.0pt;">Materials & Methods</span></em></strong><strong><span style="font-size:9.0pt;">:</span></strong><span style="font-size:9.0pt;"> Deacetylchitin nanoparticles were prepared by ionic gelation method. Zeta Sizer device measured electrical charge of nanoparticles and their size was measured by DLS and SEM. The tumor was created within two weeks after injection to BALB/c mice and then different mice groups were treated with Deacetylchitin nanoparticles and controls with PBS. After three weeks, the mice were sacrificed. The proliferation and survival of spleen lymphocyte was evaluated by MTT.</span></p> <p style="margin-left:0cm;text-align:justify;text-indent:-.1pt;line-height:18.0pt;"><strong><em><span style="font-size:9.0pt;">Results</span></em></strong><strong><span style="font-size:9.0pt;">:</span></strong><span style="font-size:9.0pt;"> Deacetylchitin nanoparticles induce proliferation of spleen cells culture. Lymphocyte proliferation showed a significant increase in Deacetylchitin nanoparticles of treated group compared to control (p <0.05).</span></p> <p style="margin-left:0cm;text-align:justify;text-indent:-.1pt;line-height:18.0pt;"><strong><em><span style="font-size:9.0pt;">Conclusion</span></em></strong>: <span style="font-size:9.0pt;">Our findings suggest that chitosan nanoparticles can stimulate the immune system and proliferate lymphocytes. This combination can be used as a medicinal supplement to stimulate the immune system to be effective in immunotherapy.</span></p> <p><strong><em>Background &&nbsp;Aims</em></strong>: Breast cancer is the most common carcinoma in women and one of the main causes of death in developed and developing countries. Today, compounds with immunolodulator properties can be replaced with routine drugs. One of them is Deacetylchitin. This study aimed to evaluate proliferation and survival of spleen immune cells treated by Deacetylchitin nanoparticles on breast cancer mouse model.</p> <p><strong><em>Materials & Methods</em></strong><strong>:</strong> Deacetylchitin nanoparticles were prepared by ionic gelation method. Zeta Sizer device measured electrical charge of nanoparticles and their size was measured by DLS and SEM. The tumor was created within two weeks after injection to BALB/c mice and then different mice groups were treated with Deacetylchitin nanoparticles and controls with PBS. After three weeks, the mice were sacrificed. The proliferation and survival of spleen lymphocyte was evaluated by MTT.</p> <p><strong><em>Results</em></strong><strong>:</strong> Deacetylchitin nanoparticles induce proliferation of spleen cells culture. Lymphocyte proliferation showed a significant increase in Deacetylchitin nanoparticles of treated group compared to control (p <0.05).</p> <p><strong><em>Conclusion</em></strong>: Our findings suggest that chitosan nanoparticles can stimulate the immune system and proliferate lymphocytes. This combination can be used as a medicinal supplement to stimulate the immune system to be effective in immunotherapy.</p> Immunotherapy, Deacetylchitin, Breast cancer Immunotherapy, chitosan nanoparticle, Breast cancer. 33 39 http://umj.umsu.ac.ir/browse.php?a_code=A-10-2436-1&slc_lang=en&sid=1 Neda Soleimani Neda Soleimani soleimani_neda86@yahoo.com 3700319475328460018738 3700319475328460018738 Yes Shahid Beheshti University Shahid Beheshti University ashraf mohabbati mobarez ashraf mohabbati mobarez ariaii39@yahoo.comll 3700319475328460018739 3700319475328460018739 No Department of bacteriology, Tarbiat modares University, Tehran, Iran Department of bacteriology, Tarbiat modares University, Tehran, Iran nima khodamabadi nima khodamabadi khoramshadi@yahoo.com 3700319475328460018740 3700319475328460018740 No Department of bacteriology, Tarbiat modares University, Tehran, Iran Department of bacteriology, Tarbiat modares University, Tehran, Iran