en Separation of ‎STIGMA STEROL using magnetic molecularly imprinted nanopolymer fabricated by sol-gel method داروسازی داروسازی پژوهشي(توصیفی- تحلیلی) Research <div style="text-align: left;"><a name="_Toc416081061"><strong><em><span style="font-size:9.0pt;">Background &&nbsp;Aims</span></em></strong></a>: <span style="font-size:9.0pt;">Magnetically molecularly imprinted polymers (MMIPs) are assumed as kind of sorbent polymers </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">which can separate or determine bioactive compounds from environment fast and specifically.&nbsp; </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">Magnetic properties, stability at various conditions (temperature , ionic strength and pH) and selective </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">function are among the advantages of these polymers in determination of nutraceutical compound. In current research, a molecularly imprinted polymer synthesized by application of Fe3O4@SiO2 nanoparticles and its functional properties were </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">evaluated.</span><br> <strong><em><span style="font-size:9.0pt;">Materials & Methods</span></em></strong><span style="font-size:9.0pt;">: In order to fabricate polymer, firstly Co</span><span dir="RTL"><span style="font-size:9.0pt;">&rlm;-&rlm;</span></span><span style="font-size:9.0pt;">precipitation method was used for </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">manufacturing of magnetic Fe3O4 nanoparticles coated by </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">silica. Then stigmasterol imprinted </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">polymer were prepared by grafting sol-gel procedure. </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">Finally obtained polymer were eluted by </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">mixture of ethanol-water-chloroform to create specific sorbent cavities for stigmasterol. Polymer </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">was incubated with stigmasterol stock solution and binding capacity </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">was determined through high </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">performance liquid chromatography.</span><br> <strong><em><span style="font-size:9.0pt;">Results</span></em></strong><span style="font-size:9.0pt;">: Structure and morphology of </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">samples were evaluated by FT-</span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">IR spectroscopy and scanning electron microscopy and Zeta sizer was used for determination of their zeta potential. </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">MMIPs </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">could separate </span><span style="font-size:9.0pt;">&lrm;<span dir="RTL">&rlm;</span></span><span style="font-size:9.0pt;">78</span><span dir="RTL"><span style="font-size:9.0pt;">% &rlm;</span></span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;"> of stigmasterol from stock solution during 60 minutes and its binding </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">capacity was </span><span style="font-size:9.0pt;">&lrm;<span dir="RTL">&rlm;</span></span><span style="font-size:9.0pt;">19.5</span><span dir="RTL"><span style="font-size:9.0pt;">&rlm;</span></span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;"> mg/g. FT-IR spectrometry and zeta potential data revealed well-designed coating </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">of silica around magnetic Fe3O4</span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;"> cores. Adsorbent silica layers were reinforced through sol-gel </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">polymerization method. Polymer morphology was porous, coarse and particle dimensions were </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;"> less than 50 nanometers.</span><br> <strong><em><span style="font-size:9.0pt;">Conclusion</span></em></strong><span style="font-size:9.0pt;">: So regarding separation and structural characteristics of properties these sorbents, the produced magnetically imprinted nanopolymer can be used for detection of stigmasterol as a neutraceutical compound.</span></div> <a name="_Toc416081061"><strong><em><span style="font-size:9.0pt;">Background &&nbsp;Aims</span></em></strong></a>: <span style="font-size:9.0pt;">Magnetically molecularly imprinted polymers (MMIPs) are assumed as kind of sorbent polymers </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">which can separate or determine bioactive compounds from environment fast and specifically.&nbsp; </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">Magnetic properties, stability at various conditions (temperature , ionic strength and pH) and selective </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">function are among the advantages of these polymers in determination of nutraceutical compound. In current research, a molecularly imprinted polymer synthesized by application of Fe3O4@SiO2 nanoparticles and its functional properties were </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">evaluated.</span><br> <strong><em><span style="font-size:9.0pt;">Materials & Methods</span></em></strong><span style="font-size:9.0pt;">: In order to fabricate polymer, firstly Co</span><span dir="RTL"><span style="font-size:9.0pt;">&rlm;-&rlm;</span></span><span style="font-size:9.0pt;">precipitation method was used for </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">manufacturing of magnetic Fe3O4 nanoparticles coated by </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">silica. Then stigmasterol imprinted </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">polymer were prepared by grafting sol-gel procedure. </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">Finally obtained polymer were eluted by </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">mixture of ethanol-water-chloroform to create specific sorbent cavities for stigmasterol. Polymer </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">was incubated with stigmasterol stock solution and binding capacity </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">was determined through high </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">performance liquid chromatography.</span><br> <strong><em><span style="font-size:9.0pt;">Results</span></em></strong><span style="font-size:9.0pt;">: Structure and morphology of </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">samples were evaluated by FT-</span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">IR spectroscopy and scanning electron microscopy and Zeta sizer was used for determination of their zeta potential. </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">MMIPs </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">could separate </span><span style="font-size:9.0pt;">&lrm;<span dir="RTL">&rlm;</span></span><span style="font-size:9.0pt;">78</span><span dir="RTL"><span style="font-size:9.0pt;">% &rlm;</span></span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;"> of stigmasterol from stock solution during 60 minutes and its binding </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">capacity was </span><span style="font-size:9.0pt;">&lrm;<span dir="RTL">&rlm;</span></span><span style="font-size:9.0pt;">19.5</span><span dir="RTL"><span style="font-size:9.0pt;">&rlm;</span></span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;"> mg/g. FT-IR spectrometry and zeta potential data revealed well-designed coating </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">of silica around magnetic Fe3O4</span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;"> cores. Adsorbent silica layers were reinforced through sol-gel </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;">polymerization method. Polymer morphology was porous, coarse and particle dimensions were </span><span style="font-size:9.0pt;">&lrm;</span><span style="font-size:9.0pt;"> less than 50 nanometers.</span><br> <strong><em><span style="font-size:9.0pt;">Conclusion</span></em></strong><span style="font-size:9.0pt;">: So regarding separation and structural characteristics of properties these sorbents, the produced magnetically imprinted nanopolymer can be used for detection of stigmasterol as a neutraceutical compound.</span> Stigmasterol, Molecularly imprinted polymer, magnetic Fe3O4@SiO2 ‎ Stigmasterol, Molecularly imprinted polymer, magnetic Fe3O4@SiO2 ‎ 44 53 http://umj.umsu.ac.ir/browse.php?a_code=A-10-2337-3&slc_lang=en&sid=1 Ehsan Moghaddas Kia Ehsan Moghaddas Kia ehsan.m.kia@gmail.com 3700319475328460018732 3700319475328460018732 No Urmia University Urmia University Mohammad Alizadeh Mohammad Alizadeh malizadeh@outlook.com 3700319475328460018733 3700319475328460018733 Yes Urmia University Urmia University Mohammad Reza Vardast Mohammad Reza Vardast mrvardast@gmail.com 3700319475328460018734 3700319475328460018734 No Urmia Medical science University Urmia Medical science University Mahmoud Rezazad Mahmoud Rezazad mahmoud114@hotmail.com 3700319475328460018735 3700319475328460018735 No Urmia University Urmia University