fa تشخیص سریع حساسیت به اتامبوتول در سویه‌های مایکوباکتریوم توبرکلوزیس ایزوله شده از بیماران مسلول با روش مولکولی PCR-RFLP آناتومی آناتومی پژوهشي(توصیفی- تحلیلی) Research <p class="Chekide" style="TEXT-JUSTIFY: kashida TEXT-ALIGN: justify TEXT-KASHIDA: 0% MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: rtl" dir="rtl"><b><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">پیش زمینه و هدف: </span></b><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">اتامبوتول یکی</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">از</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">داروهای</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">کلیدی</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">جهت</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">درمان</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">بیماری</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">سل</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">بوده</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">و</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">بروز</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">مقاومت</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">به</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">آن</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">به صورت</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">فزاینده‌ای</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">گزارش می‌شود</span><span dir="ltr"></span><span lang="FR" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2">.</font></span><span dir="rtl"></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span> در</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">مطالعه</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">حاضر</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">روش </span><font size="2"><font face="Times New Roman"><span dir="ltr">Polymorphism)</span><span dir="rtl"></span></font></font><span style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span> </span><span dir="ltr"></span><span dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2">(Restriction Fragment Length</font></span><font size="2"><font face="Times New Roman"><span lang="FR" style="mso-ansi-language: FR" dir="ltr"> PCR-RFLP</span><span dir="rtl"></span></font></font><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span> در</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">شناسایی</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">سریع</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">مقاومت</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">سویه‌های</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">مایکوباکتریوم</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">توبرکلوزیس</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">نسبت</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">به</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">اتامبوتول</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">مورد بررسی</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">قرار</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">گرفته</span><span dir="ltr"></span><span lang="AR-SA" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">است</span><span dir="ltr"></span><span lang="FR" style="mso-ansi-language: FR" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2">.</font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><p></p></span></p><p class="Chekide" style="TEXT-JUSTIFY: kashida TEXT-ALIGN: justify TEXT-KASHIDA: 0% MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: rtl" dir="rtl"><b><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">روش بررسی: </span></b><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">در</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">این</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">مطالعه از</span><span dir="ltr"></span><span dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2">127</font></span><span dir="rtl"></span><span style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span> <span lang="AR-SA"><font face="Nazanin" size="2">سویه</font></span></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">مایکوباکتریوم</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">توبرکلوزیس در</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">مرکز تحقیقات سل اراک، 60 سویه انتخاب و</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">مورد بررسی قرار گرفتند. جداسازی</span><span dir="ltr"></span><span dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> DNA </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">با استفاده از روش</span><span dir="ltr"></span><span dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> Chelex100</font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">انجام گردید</span><span lang="FA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-bidi-language: FA mso-ansi-font-size: 8.5pt">.</span><span lang="FA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"> </span><font size="2"><font face="Times New Roman"><span dir="ltr">PCR</span><span dir="rtl"></span></font></font><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span> با کمک پرایمرهای اختصاصی برای آمپلی فیکاسیون قطعه </span><font size="2"><font face="Times New Roman"><span dir="ltr">bp</span><span dir="rtl"></span></font></font><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span>167 ژن </span><font size="2"><font face="Times New Roman"><span dir="ltr">embB</span><span dir="rtl"></span></font></font><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span> اجرا گردید. محصول </span><font size="2"><font face="Times New Roman"><span dir="ltr">PCR</span><span dir="rtl"></span></font></font><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span> با کمک</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">آنزیم‌های </span><font size="2"><font face="Times New Roman"><span dir="ltr">HaeIII</span><span dir="rtl"></span></font></font><span style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span> <span lang="AR-SA"><font face="Nazanin" size="2">و </font></span></span><font size="2"><font face="Times New Roman"><span dir="ltr">NlaII</span><span dir="rtl"></span></font></font><span style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span> <span lang="AR-SA"><font face="Nazanin" size="2">مورد </font></span></span><font size="2"><font face="Times New Roman"><span dir="ltr">RFLP</span><span dir="rtl"></span></font></font><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span> قرار گرفت و الگوی باندها تعیین شد.</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">قطعه مورد نظر در تعدادی از نمونه‌ها تعیین ترادف (سکوئنس) شده و به‌عنوان استاندارد طلایی با روش ارائه شده مقایسه گردید.<p></p></span></p><p class="Chekide" style="TEXT-JUSTIFY: kashida TEXT-ALIGN: justify TEXT-KASHIDA: 0% MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: rtl" dir="rtl"><b><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">یافته‌ها: </span></b><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">از 60 سویه مورد مطالعه، 43 سویه از نظر فنوتیپی مقاوم به اتامبوتول و 17 سویه حساس به این دارو بودند. انجام </span><font size="2"><font face="Times New Roman"><span dir="ltr">PCR</span><span dir="rtl"></span></font></font><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span>، باند 167 را ارائه نمود که نشان‌دهنده انتخاب صحیح پرایمرها و تعیین برنامه مناسب آمپلی فیکاسیون بود. از 43 سویه مقاوم، 19 سویه با روش </span><font size="2"><font face="Times New Roman"><span dir="ltr">RFLP</span><span dir="rtl"></span></font></font><span style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span> <span lang="AR-SA"><font face="Nazanin" size="2">دارای موتاسیون در کدون </font></span></span><font size="2"><font face="Times New Roman"><span dir="ltr">ATG-Met</span><span dir="rtl"></span></font></font><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span> 306 و 24 سویه غیر موتان تشخیص داده شدند. تمامی 17 سویه حساس، غیر موتان بودند. این روش برای تعیین سویه حساس دارای حساسیت و ویژگی 100درصد می‌باشد. نتایج تعیین توالی، انطباق کامل </span><span lang="FA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-bidi-language: FA mso-ansi-font-size: 8.5pt">با نتایج </span><font size="2"><font face="Times New Roman"><span dir="ltr">PCR-RFLP</span><span dir="rtl"></span></font></font><span lang="FA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-bidi-language: FA mso-ansi-font-size: 8.5pt"><span dir="rtl"></span> را نشان داد.</span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><p></p></span></p><p class="Chekide" style="TEXT-JUSTIFY: kashida TEXT-ALIGN: justify TEXT-KASHIDA: 0% MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: rtl" dir="rtl"><b><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">نتیجه گیری: </span></b><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">نتایج</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">مطالعه</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">حاضر</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">نشان</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">می‌دهد</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">که</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">روش </span><font size="2"><font face="Times New Roman"><span dir="ltr">PCR-RFLP</span><span dir="rtl"></span></font></font><span style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"><span dir="rtl"></span> <span lang="AR-SA"><font face="Nazanin" size="2">می‌تواند</font></span></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">به</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">عنوان</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">یک</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">روش</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">ساده</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">و</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">سریع</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">برای</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">تعیین</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">حساسیت</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">به اتامبوتول</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">در</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">سویه‌های</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">مایکوباکتریوم</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">توبرکلوزیس</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">مورد</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">استفاده</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">قرار</span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><font face="Times New Roman" size="2"> </font></span><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt">گیرد.<p></p></span></p><p class="Chekide" style="TEXT-JUSTIFY: kashida TEXT-ALIGN: justify TEXT-KASHIDA: 0% MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: rtl" dir="rtl"><b><span lang="AR-SA" style="FONT-FAMILY: Nazanin FONT-SIZE: 9.5pt mso-ansi-font-size: 8.5pt"></span></b></p> <p>  <strong></strong></p><p> <i> <strong>Background & Aims</strong> </i>: Eethambutol is a key drug to treatment of tuberculosis and resistance against it has been increasingly reported. In this study, Polymerase Chain Reaction -Restriction Fragment Length Polymorphism (PCR-RFLP) method in rapid detection of tuberculoses mycobacterium strains against the ethambutol has been investigated. </p><p> <strong><i> Materials & Methods </i></strong>: This study was conducted on 60 strains that were selected from 127 strains in Tuberculosis and Pediatric Infections Research Cente r . DNA was extracted by Chelex 100 method. PCR test was performed using specific primers for embB gene. Digestions of PCR products with HaeIII and NlaII restriction enzymes, then the pattern of restriction fragments generated were analyzed. The section was sequenced in a few samples, and it was compared to the presented method as golden standard. </p><p> <strong><i> Results </i></strong>: Out of 60 studied stains, 43 were phenotypically resistant to ethambutol, and 17 were sensitive. PCR revealed that the band 167 showed the correct selection of primers and the appropriate plan of amplification. Out of 43 resistant strains, 19 stains were diagnosed to have mutation in ATG-Met codon 360 using RFLP method, and 24 were found to be non-mutant. </p><p> <strong><i> Conclusion </i></strong>: According to the findings, that PCR-RFLP can be a simple and rapid method to diagnose the ethambutol -sensitivity in tuberculosis mycobacterium strains. </p><p>  SOURCE: URMIA MED J 2013: 24(8): 576 ISSN: 1027-3727 </p> اتامبوتول، مایکوباکتریوم توبرکلوزیس، PCR-RFLP Ethambutol, Mycobacterium Ttuberculosis, PCR- RFLP 566 576 http://umj.umsu.ac.ir/browse.php?a_code=A-10-582-390&slc_lang=fa&sid=1 Masumeh Taherahmadi معصومه طاهراحمدی 370031947532846007880 370031947532846007880 No Department of Microbiology, Qom Branch, Islamic Azad University, دانشگاه آزاد اسلامی واحد قم، گروه میکروبیولوژی Azam Ahmadi اعظم احمدی 370031947532846007881 370031947532846007881 No Molecular and Medicine Research Center, Arak University of Medical Sciences, مرکز تحقیقات سل و عفونی کودکان، دانشگاه علوم پزشکی اراک Mana Shojapour مانا شجاع‌پور 370031947532846007882 370031947532846007882 No Molecular and Medicine Research Center, Arak University of Medical Sciences مرکز تحقیقات پزشکی و مولکولی، دانشگاه علوم پزشکی اراک Raziye Nazari راضیه نظری 370031947532846007883 370031947532846007883 No Department of Microbiology, Qom Branch, Islamic Azad University دانشگاه آزاد اسلامی واحد قم، گروه میکروبیولوژی Seyed Reza Moadab سیدرضا مودب 370031947532846007884 370031947532846007884 No Tuberculosis and Lung Research Center, Tabriz University of Medical Sciences دانشگاه علوم پزشکی تبریز Mohammad Arjomanzadegan محمد ارجمندزادگان mmatinam81@yahoo.com 370031947532846007885 370031947532846007885 Yes Tuberculosis and Pediatric Infections Research Center, Arak University of Medical Sciences مرکز تحقیقات سل و عفونی کودکان و گروه میکروبیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی اراک