TY - JOUR JF - URMIAMJ JO - Studies in Medical Sciences VL - 31 IS - 12 PY - 2021 Y1 - 2021/2/01 TI - IN-VITRO ASSESSMENT OF RESISTANCE TO ALBENDAZOLE AND FENBENDAZOLE IN GASTROINTESTINAL NEMATODES TT - مطالعه مقاومت نماتودهای دستگاه گوارش به آلبندازول و فنبندازول در شرایط آزمایشگاهی N2 - Background & Aims: Drug resistance is a great concern worldwide and in Iran. This study was carried out to assess drug resistance of gastrointestinal nematodes to Albendazole (Alb) and Fenbendazole (Feb) in sheep. Materials & Methods: A total number of 90 fresh fecal samples were directly collected from the rectum of infected sheep with an average egg per gram of feces (EPG)≤150. A dilution of 1000 eggs per 2mL was added to each well of control, Alb (0.1µg/ml) and Feb (0.1µg/ml) groups and incubated. To determine drug resistance, a lethal dose of 50% (LD50) was calculated based on percentage of the hatched eggs and first larvae stage (L1) counting. Results: EPG was lower in Feb treated groups (43.76±1.73) than Alb treated groups (65.51±1.4) and control group (93.96±0.76). There was a significant difference between percentage of the hatched eggs and both treated and control groups. LC50 demonstrated resistance to Alb in treated groups; while it uncovered suspicion to drug resistance in Feb treated groups. Conclusion: It was concluded that there was resistance to Alb and suspected resistance to Feb in sheep examined. SP - 921 EP - 926 AU - Ebrahimi, Rezgar AU - Yakhchali, Mohammad AU - Malekinejad, Hassan AD - Professor, Department of Pathobiology, Parasitology Division, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. (Corresponding Author) KW - Drug resistance KW - Albendazole KW - Fenbendazole KW - Nematodes UR - http://umj.umsu.ac.ir/article-1-4921-en.html ER -