TY - JOUR JF - URMIAMJ JO - Studies in Medical Sciences VL - 25 IS - 10 PY - 2014 Y1 - 2014/12/01 TI - THE EFFECT OF PROGESTERONE ON P53 PROTEIN IN T47D CELL LINE TT - تأثیر هورمون پروژسترون بر میزان پروتئین p53 در رده سلولی T47D سرطان پستان N2 -  Background & Aims: Breast cancer is the most common cancer in women. Nearly 50% of breast cancers are dependent to sex hormones, and the effects of these hormones are mediated by their binding to specific receptors. Also p53 protein is mutated in about half of cancers including breast cancer and high level of p53 protein is a common feature of many human malignant cancers. Given that T47D cell line has estrogen and progesterone receptor and p53 protein is product of tumor suppressor gene. This article was devoted to the effect of progesterone on p53 protein in T47D cell line. Materials & Methods: The breast cancer T47D cell line were grown in 25cm2 flasks in DMEM with fetal bovine serum (FBS). Then cells were treated with different concentrations (1 nmol, 10 nmol and 20 nmol) of progesterone hormone. The level of proteins was measured by western blot method. Gene tool software was used for data analysis. Results: There was no differences in p53 protein level in cells that were exposed to 1nmol of progesterone compared to the control group (P>0.05), but cells that were exposed to 10 and 20 nmol of progesterone treatment had lower level of p53 protein concentration than the control (P SP - 954 EP - 960 AU - Sheikhpour, Robab AU - Mohiti Ardekani, Javad AD - Department of Nursing, Yazd Medical Sciences Branch, Islamic Azad University KW - T47D cell line Breast cancer KW - Progesterone KW - p53 protein UR - http://umj.umsu.ac.ir/article-1-2551-en.html ER -