TY - JOUR JF - URMIAMJ JO - Studies in Medical Sciences VL - 25 IS - 9 PY - 2014 Y1 - 2014/11/01 TI - FOKI POLYMORPHISM, VITAMIN D RECEPTOR GENE START CODON AND ITS ASSOCIATION WITH TUBERCULOSIS TT - پلی‌مورفیسم FokI، کدون آغازین ژن گیرنده‌ی ویتامین D و ارتباط آن با بیماری سل N2 -    Background & Aims: Tuberculosis is the second factor in the deaths caused by infectious diseases in the world. Out of every ten infected people, only in one the clinical symptoms of the disease is developed. The correct vitamin D receptor activity on the nuclei of macrophages is one of the main components of the host innate immunity against M. tuberculosis. In the present study, the relationship between FokI polymorphism in the vitamin D receptor gene and tuberculosis has been investigated. Materials & Methods: The subjects were 76 patients with tuberculosis and 81 healthy controls. The genomic DNA was extracted from the blood leukocytes of all study participants, and amplified by PCR technique. Then the RFLP process was performed on all PCR products and the FokI polymorphism region was studied. The frequency of each of allele and genotypes was specified in the case and control groups and then analyzed by Chi-square test.  Results: The frequencies of FF genotype were 52.4% and 55.6% in patient and healthy groups, respectively, and also, ff genotype were 8.3% and 8.9% in patient and healthy groups (OR= 0.99, 95% CI 0.33 – 2.96, P= 0.99). These differences in frequency between them were not statistically significant. Conclusion: In this study, there was no statistically significant relationship between allelic and genotype frequency of FokI (F/f) polymorphism in VDR gene and susceptibility to tuberculosis in the respective statistical society.   SOURCE: URMIA MED J 2014: 25(9): 783 ISSN: 1027-3727 SP - 778 EP - 783 AU - Asgharzadeh, Mohamad AU - Rashedi, Jalil AU - Moaddab, Seyyed Reza AU - Ansarin, Khalil AU - Mirgasemi, Zahra AU - Shirazi, Samaneh AD - Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences KW - Tuberculosis KW - Vitamin D KW - Polymorphism KW - Genetics UR - http://umj.umsu.ac.ir/article-1-2487-en.html ER -