AU - Ahangarpour, Akram AU - Oroojan, Ali Akbar AU - Heydari, Hamid AU - Ahmadi, Iraj TI - EFFECTS OF AQUEOUS AND HYDRO-ALCOHOLIC EXTRACTS OF BUNIUM PERSICUM SEED ON INSULIN SECRETION FROM MALE MOUSE-ISOLATED LANGERHANS ISLETS PT - JOURNAL ARTICLE TA - URMIAMJ JN - URMIAMJ VO - 25 VI - 8 IP - 8 4099 - http://umj.umsu.ac.ir/article-1-2449-en.html 4100 - http://umj.umsu.ac.ir/article-1-2449-en.pdf SO - URMIAMJ 8 AB  -  Background & Aims: As B. persicum is used in treatment of diabetes, we aimed to study the effect of B. persicum seeds extract on level of insulin secretion from isolated islets of langerhans in male mouse.Materials & Methods: In this experimental study, adult male mice (NMARI) weighting 20-25g were purchased. Islets of langerhans were isolated by collagenase digestion method and were divided into 18 groups: (glucose2.8mM and 16.7mM), aqueous and hydro-alcoholic extracts of B. persicum seeds (0.05, 0.1, 1mg/ml) and glyburide (1, 10μM). Each concentration of extracts and glyburide applied separately on isolated islets. Insulin secretion from isolated islets was evaluated by a static incubation system, and insulin secretion amount was measured by IRMA method.Results: Amount of insulin secretion in 0.05, 0.1mg/ml concentrations of B. persicum seed extracts in mediums containing glucose2.8 mM and 16.7 mM increased significantly in comparison with glucose2.8 mM and 16.7 mM groups. 0.05mg/ml of aqueous extract in mediums containing glucose 2.8 mM and 16.7 mM increased insulin secretion when compared to various concentrations of hydro-alcoholic extracts. also among all groups, glyburide10μM showed most effective in increasing insulin secretion from isolated islets in comparison with glucose2.8mM and 16.7mM groups (p CP - IRAN IN - Ahvaz, Ahvaz Jundishapur University of Medical Sciences, Faculty of Medicine, Department of Physiology, Tel: 09166059217 LG - eng PB - URMIAMJ PG - 742 PT - Research YR - 2014