TY - JOUR JF - URMIAMJ JO - Studies in Medical Sciences VL - 25 IS - 2 PY - 2014 Y1 - 2014/5/01 TI - PREVENTION EFFECT OF TURMERIC EXTRACT ON METHOTREXATE-INDUCED INTESTINAL TOXICITY BY ALLEVIATING OXIDATIVE STRESS IN RATS TT - تأثیر حفاظتی عصاره اتانولی زردچوبه بر آسیب‌های روده القا شده توسط متوترکسات با کاهش استرس اکسیداتیو در موش صحرایی N2 -    Background & Aims: The aim of the present study was to evaluate the protective effects of turmeric on intestinal damage and oxidative stress induced by Methotrexate. Materials & Methods: For this purpose, male rats were divided into 6 groups. Group I received normal saline orally. Group II received single dose of methoterxate (20 mgkg -1) IP at day of 30, the fifth and the sixth groups received turmeric extract received different dose of turmeric and single dose of methoterxate (MTX). Four days after MTX injection blood samples were taken to evaluation the total antioxidant status and jejunum samples were taken to determine antioxidant enzyme activities, aldehyde malondialdehyde (MDA), and histopathological assessment.  Results: The histopathology analysis showed that the mean difference between groups II received single dose of methotrexate and groups V and VI received methotrexate plus turmeric. There are epithelial cells necrosis, inflammatory cells, blunting and villus shortening in second group. However in groups V and VI have been shown to be ameliorated and decreased in pathologic results, especially in group VI. Biochemical result shown that the levels of superoxidase dismutase, glutathione peroxidase and catalase reduced in group II, but increased significantly (P< 0.05) in turmeric + MTX groups. MTX increased lipid peroxidation however, turmeric decreased peroxidation significantly (P SP - 119 EP - 128 AU - Mazani, Mohammad AU - Tutunchi, Soheil AU - Shahi, Delavar AU - Manafi, Hamed AU - Yazdi, Mina AU - Khajoie Najad, Marzieh AU - Rezaei Moghadam, Adel AU - Mohajeri, Daryoush AD - Department of Biochemistry, Ardabil University of Medical Science KW - Turmeric extract KW - Methoterxate KW - Intestinal KW - Oxidative stress KW - Rat UR - http://umj.umsu.ac.ir/article-1-2158-en.html ER -