TY - JOUR JF - URMIAMJ JO - Studies in Medical Sciences VL - 27 IS - 10 PY - 2017 Y1 - 2017/1/01 TI - Role of mexZ gene in ciprofloxacin resistance in Pseudomonas aeruginosa isolates in Guilan province TT - نقش ژن mexZ در مقاومت به سیپروفلوکساسین در جدایه های سودوموناس آئروژینوزا در استان گیلان N2 - Messadi AA, Lamia T, Kamel B, Salima O, Monia M, Saida BR. Association between antibiotic use and changes in susceptibility patterns of Pseudomonas aeruginosa in an intensive care burn unit: a 5-year study, 2000-2004. Burns :J Int Soc Burn Injuries 2008;34(8):1098-102. Sherertz RJ, Sarubbi FA. A three-year study of nosocomial infections associated with Pseudomonas aeruginosa. J Clin Microbiol 1983;18(1):160-4. Henrichfreise B, Wiegand I, Pfister W, Wiedemann B. Resistance mechanisms of multiresistant Pseudomonas aeruginosa strains from Germany and correlation with hypermutation. Antimicrob Agents Chemother 2007;51(11):4062-70. Cole SJ, Records AR, Orr MW, Linden SB, Lee VT. 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Role of MexZ and PA5471 in transcriptional regulation of mexXY in Pseudomonas aeruginosa. Microbiol 2009;155(Pt 10):3312-21. Hocquet D, Berthelot P, Roussel-Delvallez M, Favre R, Jeannot K, Bajolet O, et al. Pseudomonas aeruginosa may accumulate drug resistance mechanisms without losing its ability to cause bloodstream infections. Antimicrob Agents Chemother 2007;51(10):3531-6. Geyik MF, Aldemir M, Hosoglu S, Tacyildiz HI. Epidemiology of burn unit infections in children. Am J Infection Control 2003;31(6):342-6. Llanes C, Hocquet D, Vogne C, Benali-Baitich D, Neuwirth C, Plesiat P. Clinical strains of Pseudomonas aeruginosa overproducing MexAB-OprM and MexXY efflux pumps simultaneously. Antimicrob Agents Chemother 2004;48(5):1797-802. Background & Aims: Pseudomonas aeruginosa is an opportunistic pathogen and one of mortality causes of nosocomial infections specifically in severely burned patients. One of the drug resistant mechanisms in pseudomonas aeruginosa is mutation in negative regulator genes of mexXY efflux pump system. In this study, the role of mexZ mutations was investigated in ciprofloxacin resistant development in Pseudomonas aeruginosa isolates in Guilan province. Materials & Methods: In this study, 45 strains of pseudomonas aeruginosa isolated from different clinical samples of Rasht and Lahijan hospitals and laboratories between 2014 to 2016 were identified by biochemical tests. The antibiotic resistance and susceptibility of the strains were investigated by Kirby Bauer method and MIC determination. Then PCR-sequencing was performed to assess MexZ gene mutations in ciprofloxacin resistant strains. Results: From 45 isolates of pseudomonas aeruginosa, all were resistant to cefixime, cephalothin and trimethoprim; whereas 17 isolates were ciprofloxacin resistant. The highest MIC of ciprofloxacin was determined 1024 µg/ml. Also, PCR-sequencing analysis showed that 8 isolates had missense mutations in MexZ gene such as L111E and R143P. Conclusion: In this study, mutation in mexZ as negative regulator of mexXY can be a reason of multi-drug resistance in some strains in Guilan province. It appears that mexZ mutation led to affinity modification in mexZ protein binding to DNA in some isolates. SOURCE: URMIA MED J 2016: 26(10): 913 ISSN: 1027-3727 SP - 902 EP - 913 AU - Ranji, Najmeh AU - Rahbar Takrami, Saeid AD - Rasht Branch, Islamic Azad University KW - Ciprofloxacin KW - MexXY KW - MexZ KW - Pseudomonas Aeruginosa UR - http://umj.umsu.ac.ir/article-1-3559-en.html ER -