TY - JOUR JF - URMIAMJ JO - Studies in Medical Sciences VL - 27 IS - 5 PY - 2016 Y1 - 2016/8/01 TI - EVALUATION OF GENETIC DIVERSITY OF SALMONELLA ENTERICA SEROVAR ENTERITIDIS ISOLATED FROM HUMAN AND ANIMALS SAMPLES BY ERIC-PCR TT - ارزیابی ارتباط ژنتیکی سالمونلا انتریکا سرووار انتریتیدیس جدا شده از نمونه ‏های انسانی و دامی به روش ‏ERIC- PCR N2 - Background & Aims: Salmonellosis is one of the vital infectious zoonotic diseases in both human and animals that is related with poultry, meat, egg and milk consumption. The aim of this study was evaluation of genetic diversity of Salmonellaenterica serovar enteritidis isolated from human and animals samples by ERIC-PCR method. Materials & Methods: In this cross-sectional study, 60 Salmonellaenterica serovar enteritidis were obtained from the human and animals. Detection of strains were performed by standard microbiological and biochemical tests and slid agglutination assay were done for serotyping of the strains by mono and polyvalent antisera. Then, ERIC-PCR was achieved for determination of molecular correlation of the strains by specific oligonucleotides primers. Results: The results showed that, all 60 S. enterica using ERIC 1 and ERIC were type II. And 2-11 bands with 20-3200 bp were obtained. Therefore, 15 different clusters (C1-C15) were attained and that highest number (13.3%, 8 strains) with like pattern were in cluster 5 (C5). Conclusion: The results showed that Salmonellaenterica serovar enteritidis strains are non-homolog. So, ERIC-PCR method is an appropriate method for molecular typing of Salmonella strains and infection spread source determination used for epidemiologic survey and infection prevention pathway. SOURCE: URMIA MED J 2016: 27(5): 418 ISSN: 1027-3727 SP - 411 EP - 418 AU - porhasan sangari, farzaneh AU - Amini, Kumarss AU - Moradli, Gholamali AD - Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran KW - Salmonella enterica KW - Molecular Typing KW - ERIC-PCR.‎ UR - http://umj.umsu.ac.ir/article-1-3215-en.html ER -