Volume 21, Number 4 (Dec-Nov Biomonthly 2010)                   J Urmia Univ Med Sci 2010, 21(4): 324-331 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Pajohi M R, Tajik H, Akhondzade A, Gandomi H, Ehsani A, Shokohi Sabet Jalali F. EVALUATION OF CHEMICAL COMPOSITION AND ANTIBACTERIAL EFFICACY OF CUMINUM CYMINUM L. AND MENTHA LONGIFOLIA L. ALONE AND COMBINED WITH NISIN. J Urmia Univ Med Sci. 2010; 21 (4) :324-331
URL: http://umj.umsu.ac.ir/article-1-785-en.html

Associate Professor of Food Hygiene and Quality Control , h.tajik@urmia.ac.ir
Abstract:   (26455 Views)

  Received: 25 Aug, 2010 Accepted: 16 Oct, 2010

  Abstract

  Background & A ims : Mentha longifolia and cumin seed were used for long time as spices and in conventional medicine . T his study was undertaken in order to evaluate the chemical composition of essential oils of Mentha longifolia L. and cumin seed (dried seed of the herb Cuminum cyminum L.) and their antibacterial efficacy alone and combined with nisin on Bacillus cereus and Bacillus subtilis.

  Materials & Methods : The essential oil of the Mentha longifolia L. and Cuminum cyminum L. were obtained using steam distillation method by Clevenger apparatus and their chemical composition was assed by Gas chromatography-mass spectrometry (GC/MS). Broth microdilution susceptibility assay is used to determine minimum inhibitory concentration of essential oils and nisin.

  Results : Pulegone (31.54%) and Cumin aldehyde (29.02%) were the most abundant chemicals in horse-mint and cumin seed, respectively. Mentha longifolia , cumin , and nisin showed antibacterial activity on Bacillus cereus in concentration of 0.25%, 0.25%, and 10µg/ml, respectively. The corresponding activity on Bacillus subtilis were 0.25%, 0.125%, and 25µg/ml.

  Conclusion : The combined use of these essentials oils with nisin increased their antibacterial properties substantially. Furthermore, the bacterial growth curve showed that the efficacy of cumin seed alone or combined with nisin were greater to that of Mentha longifolia. The results of this study can be employed in food industry to overcome the hazards posed by pathogenic microorganisms.

 

 

 

  Source: Urmia Med J 2010: 21(4): 379 ISSN: 1027-3727

Full-Text [PDF 295 kb]   (1650 Downloads)    
Type of Study: Research | Subject: آناتومی
Received: 2010/12/13

Add your comments about this article : Your username or email:
Write the security code in the box

Send email to the article author


© 2015 All Rights Reserved | URMIA MEDICAL JOURNAL

Designed & Developed by : Yektaweb