Volume 34, Issue 1 (April 2023)
Studies in Medical Sciences 2023, 34(1): 25-34 |
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Ethics code: IR.SHAHED.REC.1397.082
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Naseri A, Khalili M, Haddadzadeh-Niri N, Roghani M. THE EFFECT OF CROCIN ON LIVER DYSFUNCTION INDUCED BY LIPOPOLYSACCHARIDE/D-GALACTOSAMINE IN MALE MOUSE. Studies in Medical Sciences 2023; 34 (1) :25-34
URL: http://umj.umsu.ac.ir/article-1-5842-en.html
URL: http://umj.umsu.ac.ir/article-1-5842-en.html
Professor of Physiology, Neurophysiology Research Center, Shahid University, Tehran, Iran (Corresponding Author) , mehjour@yahoo.com
Abstract: (723 Views)
Background & Aim: Acute liver injury is a fatal syndrome that causes rapid loss of liver function. Crocin is the active ingredient of saffron with antioxidant, protective and anti-apoptotic properties. In this study, the protective effect of crocin was evaluated on the liver dysfunction in male laboratory mice.
Materials & Methods: In this experimental study, 28 male mice (20-24 g) were divided into control, liver damage, and two groups treated with crocin at doses of 50 or 200 mg/kg. liver damage was caused by the injection of lipopolysaccharide/D-galactosamine with doses of 50 microg/kg and 400 mg/kg, respectively. Crocin dissolved in distilled water was fed by gavage at 50 or 200 mg/kg, starting 3 days before and up to 1 hour before injury induction. 24 hours after injury induction, the mice were anesthetized with a mixture of 120 mg/kg ketamine and 10 mg/kg xylazine, and sacrificed in compliance with ethical rules after taking a blood sample from their hearts. liver markers including MDA, SOD, Cat, MPO, and TNF-α were measured. Data was analyzed with GraphPad Prism 9.3 software using one-way ANOVA and Tukey's post hoc tests with significance level set at p<0.05.
Results: Administration of crocin decreased serum levels of liver markers MDA, MPO, and TNF-α and increased SOD in crocin-treated groups as compared to the LPS/D-Gal group (p<0.05); Cat change was not significant (p>0.05).
Conclusion: Crocin with strong antioxidant activity was able to reduce liver dysfunction by reducing oxidative stress and inflammation.
Materials & Methods: In this experimental study, 28 male mice (20-24 g) were divided into control, liver damage, and two groups treated with crocin at doses of 50 or 200 mg/kg. liver damage was caused by the injection of lipopolysaccharide/D-galactosamine with doses of 50 microg/kg and 400 mg/kg, respectively. Crocin dissolved in distilled water was fed by gavage at 50 or 200 mg/kg, starting 3 days before and up to 1 hour before injury induction. 24 hours after injury induction, the mice were anesthetized with a mixture of 120 mg/kg ketamine and 10 mg/kg xylazine, and sacrificed in compliance with ethical rules after taking a blood sample from their hearts. liver markers including MDA, SOD, Cat, MPO, and TNF-α were measured. Data was analyzed with GraphPad Prism 9.3 software using one-way ANOVA and Tukey's post hoc tests with significance level set at p<0.05.
Results: Administration of crocin decreased serum levels of liver markers MDA, MPO, and TNF-α and increased SOD in crocin-treated groups as compared to the LPS/D-Gal group (p<0.05); Cat change was not significant (p>0.05).
Conclusion: Crocin with strong antioxidant activity was able to reduce liver dysfunction by reducing oxidative stress and inflammation.
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