Volume 31, Issue 9 (December 2020)
Studies in Medical Sciences 2020, 31(9): 650-657 |
Back to browse issues page
Department of Biology, Faculty of Basic Sciences, Bu- Ali Sina University, Hamedan, Iran (Corresponding Author) , mirazi205@gmail.com
Abstract: (2263 Views)
Background & Aims: The aim of this study was to evaluate the protective effects of raspberry fruit extract (Rubus fruticosus L.) on LH, FSH, testosterone serum levels, and number and percentage of sperm motility in male diabetic rats induced with streptozotocin.
Materials & Methods: In this experimental study, 50 Wistar male rats were used and divided into five groups: (control), diabetic (STZ,55mg/kg, i.p), and treatment groups (DM + raspberry fruit extract: 50, 100, 200 mg/kg/day, 4 weeks). At the end of experiments, the LH, FSH and testosterone serum levels and the number and percentage of sperm motility were examined. The data were statistically analyzed by ANOVA test.
Results: The LH and FSH serum levels increased significantly in DM group compared with other groups (p<0.05). After treatment with raspberry fruit extract, the LH and FSH serum levels decreased significantly compared with DM group (p<0.05). The testosterone serum level and the number and percentage of sperm motility decreased significantly in the DM group (p<0.05). The use of raspberry fruit extract significantly increased testosterone serum level and the number and percentage of sperm motility in treatment groups (p<0.05).
Conclusion: Our results showed that raspberry fruit extract increased testosterone hormone in DM rats, and also improved spermatogenesis and ameliorating pituitary-gonadal axis function.
Materials & Methods: In this experimental study, 50 Wistar male rats were used and divided into five groups: (control), diabetic (STZ,55mg/kg, i.p), and treatment groups (DM + raspberry fruit extract: 50, 100, 200 mg/kg/day, 4 weeks). At the end of experiments, the LH, FSH and testosterone serum levels and the number and percentage of sperm motility were examined. The data were statistically analyzed by ANOVA test.
Results: The LH and FSH serum levels increased significantly in DM group compared with other groups (p<0.05). After treatment with raspberry fruit extract, the LH and FSH serum levels decreased significantly compared with DM group (p<0.05). The testosterone serum level and the number and percentage of sperm motility decreased significantly in the DM group (p<0.05). The use of raspberry fruit extract significantly increased testosterone serum level and the number and percentage of sperm motility in treatment groups (p<0.05).
Conclusion: Our results showed that raspberry fruit extract increased testosterone hormone in DM rats, and also improved spermatogenesis and ameliorating pituitary-gonadal axis function.
References
1. Canivell S, Gomis R. Diagnosis and classification of autoimmune diabetes mellitus. Autoimmun Rev 2014; 13: 403-7. [DOI:10.1016/j.autrev.2014.01.020] [PMID]
2. Ahmadinasab M, Valipour chahardah charic S, Setorki M. Effect of Crataegus monogyna extract on renal histopathological changes in diabetic rats. Pars Journal of Medical Sciences 2019; 17: 23-31. [DOI:10.52547/jmj.17.2.23]
3. Bolbol Haghighi N, Molzemi S, Goli Sh, Mohammad Sadeghi H, Aminian M. The Effect of Hydroalcoholic Extract of Ziziphora Clinopodioides Lam on Testicular Damage Caused by Diabetes Mellitus in Male Rats. J Babol Univ Med Sci 2017; 19: 43-9. [URL]
4. Soudamani S, Yuvaraj S, Rengarajan S, Sivakumar R, Malini T, Balasubramanian K. Effects of streptozotocindiabetes and insulin replacement on androgen and estrogen receptor concentrations in the epididymis of Wistar rats. Journal of Endocrinology and Reproduction 2006; 10: 59-61. [URL]
5. Sadoughi SD, Edalatmanesh MA, Rahbarian R. The Effect of Curcumin on Pituitary‐Gonadal Axis, DNA Oxidative Damage and Antioxidant Enzymes Activity of Testicular Tissue in Male Diabetic Rats. Journal of Fasa University of Medical Sciences 2018; 7: 511-20. [Google Scholar]
6. Dadfar F, Mohammadi J, Roshanfekr H. Effect of hydroalcoholic extract of Nectaroscordum tripedale on testicular tissue changes in diabetic male rats. Feyz Sciences 2019; 23: 108-16. [URL]
7. Jin J, Yang W. Molecular regulation of hypothalamus-pituitary-gonads axis in males. Gene 2014; 551: 15-25. [DOI:10.1016/j.gene.2014.08.048] [PMID]
8. Holdcraft RW, Braun RE. Hormonal regulation ofspermatogenesis. Int J Androl 2004; 27: 335-42. [DOI:10.1111/j.1365-2605.2004.00502.x] [PMID]
9. Spark RF. Testosterone, diabetes mellitus, and themetabolic syndrome. Curr Urol Rep 2007; 8: 467-71. [DOI:10.1007/s11934-007-0050-4] [PMID]
10. Taghavi MM, Mahmoudian AR, Pourmasumi S, Jafari Naveh HR, Alavi H. The effect of Angi Pars as an herbal medicine on sperm count and the ratio of testis weight to body weight in diabetic rats. J Mazandaran Univ Med Sci 2011; 24: 68-75. [URL]
11. Stoner GD, Chen T, Kresty LA, Aziz RM, Reinemann T, Nines R. Protection against esophageal cancer in rodents with lyophilized berries: Potential mechanisms. Nutr Cancer 2006; 54: 33-46. [DOI:10.1207/s15327914nc5401_5] [PMID] [PMCID]
12. Ştefănuţ MN, Căta A, Pop R, Tănasie C, Boc D, Ienaşcu I, et al. Anti-hyperglycemic effect ofbilberry, blackberry and mulberry ultrasonic extracts on diabetic rats. Plant Foods Hum. Nutr 2013; 68: 378-84. [DOI:10.1007/s11130-013-0380-y] [PMID]
13. Yoon J, Cao X, Zhou Q, Ma LQ. Accumulation of Pb, Cu, and Zn in native plants growing on a contaminated Florida site. Sci Total Environ 2006; 368: 456-64. [DOI:10.1016/j.scitotenv.2006.01.016] [PMID]
14. Zia-Ul-Haq M, Riaz M, De Feo V, Jaafar HZ, Moga M. Rubus Fruticosus L.: Constituents, Biological Activities and Health Related Uses. Molecules 2014; 19: 10998-1029. [DOI:10.3390/molecules190810998] [PMID] [PMCID]
15. Shahmohamadi S, Khosravi M, Hajizadeh Moghaddam A. Effect of Salvia officinalis L. extract on malondialdehyde against streptozotocin-induced oxidative stress in rat's brain. Medical sciences 2014; 23: 225-9. [URL]
16. Gomar A, Hosseini A, Mirazi N, Gomar M. Effect of hydroethanolic extract of Rubus fruticosus on neuropathic pain in Wistar diabetic rats. Caspian J Neurol Sci 2015; 1: 27-34. [DOI:10.18869/acadpub.cjns.1.1.27]
17. Pakseresht Z, Norouzi P, Hojati V, Moghaddam Kalalian H. Effect of Palmatine Hydrochloride on Oxidative Stress in Streptozotocin -Induced Diabetic Rats. J Adv Med Biomed Res 2016; 24: 119-29 [URL]
18. Shiravi A, Sayyad Zomorrodi M. The study effect of rubus fruticosus extract on cutaneous wound healing in diabetic wistar rat. Iranian Journal of Diabetes and Metabolism 2015; 15: 1-8. [URL]
19. Soleimanzadeh A, Malekifard F, Kabirian A. Protective effects of hydro-alcoholic garlic extract on spermatogenic disorders in streptozotocin-induced diabetic C57BL/6 mice. Sci J Kurdistan Univ Med Sci 2017; 22: 8-17. [URL]
20. Hajinezhad MR, Shapari A, Hajian Shahri S, Sarani F, Salehimoghadam M. Effect of Hydroalcoholic Extract of Berberis Vulgaris Root on Serum Levels of Glucose, Malondehyde and HbA1c in Diabetic Rats. J Neyshabur Univ Med Sci 2015; 3: 21-8. [URL]
21. hassanvand V, Yousofvand N, Hatami K. Preventive effect of combining zinc sulphate and garlic flowers extract on diabetes induced by streptozotocin (STZ) in male rats. Journal of Animal Research (Iranian Journal o Biology) 2016; 30: 33-41. [URL]
22. Mohammadi Mehdiabadihassani MH, Moradi M, Khoshnam E, Mohammadi SH. The Effects of Hydroalcoholic Extract of Raspberry fruit on Pain and Passive Avoidance Memory Following Transient Global Ischemia/Reperfusion in Wistar Rats. J Med Plants 2017; 16: 137-46. [URL]
23. Amini N, Shiravi A, Mirazi N, Hojati V, Abbasalipourkabir R. Protective effects of the raspberry (Rubus fruticosus L.) on pituitary-gonadal axis and testicular histopathology in streptozotosin induced diabetic male rats. Avicenna journal of phytomedicine 2020; Articles in Press. [Google Scholar]
24. Natah TM, Abdul- Adheem Wtwt M, Al-Saadi HK, Al-Saadi AH, Farhood HF. Study the levels of adiponectin, FSH, LH and Sex hormones in Type 2 diabetes (NIDDM). Journal of Biology, Agriculture and Healthcare 2013; 3: 172-81. [Google Scholar]
25. Ballester J, Munoz MC, Dominguez J, Rigau T, Guinovart JJ, Rodriguez-Gil JE. Insulindependent Diabetes Affects Testicular Function by FSH-and LH Linked Mechanisms. Journal of Andrology 2004; 25: 709-19. [DOI:10.1002/j.1939-4640.2004.tb02845.x] [PMID]
26. Natah TM, Wtwt MA, Al-Saadi HK, Al-Saadi AH, Farhood HF. Study the levels of adiponectin, FSH, LH, and sex hormone in type 2 diabetes (NIIDDM). J Biol Agric Healthcare2013; 3: 172-81. [Google Scholar]
27. Sönmez MF, Karabulut D, Kilic E, Akalin H, Sakalar C, Gunduz Y, et al. The effects of streptozotocin-induced diabetes on ghrelin expression in rat testis: biochemical and immunohistochemical study. Folia Histochem Cytobiol 2015; 53: 26-34. [DOI:10.5603/FHC.a2015.0006] [PMID]
28. Sepehri H, Rastgar farajzadeh A, ghasemi K. Guyton and Hall textbook of medical Physiology. 13th ed. Tehran: Andishe rafi publications; 2016.Rubus fruticosus L [URL]
29. Najar A, Piryae A, Babaei S, bayat M. Effect of pentoxifylline on Sertoli and Leydig cells count of experimentally induced type 1 diabetes in male rats. J Army Univ Med Sci 2013; 11: 188-95. [Google Scholar]
30. Khaki A, Khaki AA, Hajhosseini L, Sadeghpour Golzar F, Ainehchi N. The anti-oxidant effects of ginger and cinnamon on spermatogenesis dys-function of diabetes rats. Afr J Tradit Complement Altern Med 2014; 11: 1-8. [DOI:10.4314/ajtcam.v11i4.1] [PMID] [PMCID]
31. Nejati V, Khaneshi F. Evaluation of hydro- alcoholic extract of Plantago majo leaf on the changes in testis Morphology, sperm parameters and testosterone level in Streptozotocin- Induced Diabetic Rats. Quarterly of Horizon of Medical Sciences 2014; 20: 49-55. [Google Scholar]
32. Jasemi M, Saki GH, Rahim F. The Effect of Centella Asiatica Alcoholic Extract on the Serum Levels of Testosterone FSH and LH in Male Wistar Rat. J Sabzevar Univ Med Sci2009; 16:6-11. [Google Scholar]
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |