Volume 32, Issue 2 (May 2021)
Studies in Medical Sciences 2021, 32(2): 92-104 |
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Azhari B, Noorbakhsh F, Eidi M. FREQUENCY OF CARBAPENEMASE AND METALLO-BETA-LACTAMASE RESISTANCE AND OXA- 48, OXA-23, AND NDM GENES IN CLINICAL ISOLATES OF ACINETOBACTER BAUMANNI IN 2017. Studies in Medical Sciences 2021; 32 (2) :92-104
URL: http://umj.umsu.ac.ir/article-1-5188-en.html
URL: http://umj.umsu.ac.ir/article-1-5188-en.html
Department of Microbiology, Biological Science College, Varamin-pishva branch, Islamic Azad University, Varamin-Pishva, Iran (Corresponding Author) , niloofar_noorbakhsh@yahoo.com
Abstract: (1923 Views)
Background & Aims: One of the most important problems in treatment centers is the infectious diseases caused by antibiotic resistant Acinetobacter Bumanni. This study aimed to isolate and identify carbapenemase and metallo-beta-lactamase producing strains using phenotypic and molecular methods.
Materials & Methods: In this study 79 strains of Acinetobacter Bumanni were isolated from patients hospitalized in Tehran Heart Hospital and identified by biochemical tests. Antibiotic susceptibility of isolates was performed by disc diffusion method. Phenotypic methods such as combined disk test (CDT), double disk synergy test (DDST), and Modified Hodge test (MHT) were performed to identify carbapenemase and metallo-beta-lactamase activity. PCR was performed using specific primers for OXA- 48, OXA-23, and NDM genes.
Results: In this study, the highest resistance in A. baumanii was observed to imipenem and ertapenem by disk diffusion method. By CDT, 96.2% of isolates showed carbapenemase activity and 94.93% showed metallo-beta-lactamase activity in presence of imipenem. Also, by DDST, 86.07% and 91.13% of isolates showed Carbapenemase and metallo-beta-lactamase activity, respectively, and 91.14% of isolates were positive by MHT. The molecular method showed that OXA-48 gene was in 100% of isolates and OXA-23 gene was in 98.73% of isolates and NDM gene did not exist in isolates.
Conclusion: Based on the results, CDT has high susceptibility in other phenotypic methods for identifying carbapenemase and metallo-beta-lactamase activity. Frequency of OXA-48 and OXA-23 genes revealed antibiotic resistance in A. baumanii isolates.
Materials & Methods: In this study 79 strains of Acinetobacter Bumanni were isolated from patients hospitalized in Tehran Heart Hospital and identified by biochemical tests. Antibiotic susceptibility of isolates was performed by disc diffusion method. Phenotypic methods such as combined disk test (CDT), double disk synergy test (DDST), and Modified Hodge test (MHT) were performed to identify carbapenemase and metallo-beta-lactamase activity. PCR was performed using specific primers for OXA- 48, OXA-23, and NDM genes.
Results: In this study, the highest resistance in A. baumanii was observed to imipenem and ertapenem by disk diffusion method. By CDT, 96.2% of isolates showed carbapenemase activity and 94.93% showed metallo-beta-lactamase activity in presence of imipenem. Also, by DDST, 86.07% and 91.13% of isolates showed Carbapenemase and metallo-beta-lactamase activity, respectively, and 91.14% of isolates were positive by MHT. The molecular method showed that OXA-48 gene was in 100% of isolates and OXA-23 gene was in 98.73% of isolates and NDM gene did not exist in isolates.
Conclusion: Based on the results, CDT has high susceptibility in other phenotypic methods for identifying carbapenemase and metallo-beta-lactamase activity. Frequency of OXA-48 and OXA-23 genes revealed antibiotic resistance in A. baumanii isolates.
Keywords: Acinetobacter baumanii, Carbapenemas, Metallo-beta-lactamase, combined disk test (CDT), double disc synergy test (DDST), Modified Hodge test (MHT)
Type of Study: Research |
Subject:
میکروبیولوژی
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