Volume 20, Number 2 (Summer 2009)                   J Urmia Univ Med Sci 2009, 20(2): 111-116 | Back to browse issues page


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A Javadirad, S H Madani, S Khazaei, M Rahbar. DIAGNOSTIC VALUE OF AgNOR STAINING AND KI-67 (MIB1) EXPRESSION IN UTERINE SMOOTH MUSCLE TUMORS. J Urmia Univ Med Sci. 2009; 20 (2) :111-116
URL: http://umj.umsu.ac.ir/article-1-431-en.html

Assistant Professor of Pathology, Kermanshah University of Medical Sciences, Kermanshah, Iran , shmmadani@yahoo.com
Abstract:   (14919 Views)

  Abstract

 Background & Aims: Uterine smooth muscle tumors are the most common human neoplasm. They are divided clinically as benign and malignant but there is another group of lesions which is difficult to place in these two categories, so-called STUMP and differentiation of these tumors on the basis of H&E staining is difficult. The goal of this study is to find distinguishing objective biomarker and a survey of proliferation markers comparing these three groups of tumors.

 Materials &Methods: Twenty one cases in each group of the above mentioned tumors were selected randomly from pathobiology laboratories and studied by using IHC staining for Ki_67 expression and AgNOR method.

 Results: Ki_67 was expressed in 63.15% of leiomyosarcomas, 4.76% of STUMPs and 0% of leiomyoma group. Ki_67 expression between leiomyosarcoma and STUMP, and also between leiomyosarcom and leiomyoma were significant (P<0.0001). Mean AgNOR dots were 2.55 ± 0.03, 4.04 ± 0.66 and 8.12 ± 0.13 in leiomyoma, STUMP and leiomyosarcoma, respectively. Significant differences between three groups were observed (P<0.0001).

 Conclusion: AgNOR and Ki_67 (MIB1) proliferation markers expression between leiomyosarcoma and STUMP showed significant differences. Due to difficulty in differention of leiomyosarcoma from STUMP, finding objective biomarker is necessary. For this purpose, the present study recommends Ki_67 and AgNOR staining for this purpose and seems to be a reliable, simple, and rapid method.

 

 Keywords: Nucleolar organizer regions, AgNOR, Uterine smooth muscle tumor, Ki_67

 

 Address:Pathology Department, Kermanshah University of Medical Sciences, Kermanshah, Iran
Tel : (+98) 09188329445

 

  E-mail: shmmadani@yahoo.com

 

  Source: UMJ 2009: 20(2): 157 ISSN: 1027-3727

Full-Text [PDF 255 kb]   (1245 Downloads)    
Type of Study: Research | Subject: آناتومی
Received: 2009/09/2

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