Volume 28, Number 3 (Monthly_Jun 2017)                   J Urmia Univ Med Sci 2017, 28(3): 173-181 | Back to browse issues page


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Mohammadi R, Moradi M, tajik H, Ghasemmahdi H, Modaresi R, Khalili Sadaghiani S. EFFECT OF GROWTH TEMPERATURE AND BIOFILM AGE ON THE RESISTANCE OF SALMONELLA TYPHIMURIUM BIOFILMS TO BACTERIOPHAGE . J Urmia Univ Med Sci. 2017; 28 (3) :173-181
URL: http://umj.umsu.ac.ir/article-1-3716-en.html

Assistant Professor Urmia University , moradi.mehran@yahoo.com
Abstract:   (557 Views)

Background & Aims: Salmonella Typhimurium is among the most important food-borne disease. This study was conducted to investigate the anti-biofilm effect of the S. Typhimurium phage against Salmonella formed in chicken meat model.

Materials & Methods: The effects of different phage concentrations (104, 106 and 108 PFU/mL) with two contact times (10 and 20 min) on one and 7 days old biofilm adhered to stainless steel coupon in chicken broth at 15, 8 and 4 °C were evaluated.

Results: The results showed that the bacteria had an ability to adhere to the coupons and form biofilm in chicken broth, the biofilm levels were significantly (0.05 ≥ P) higher at 15 °C for 7 days. Increasing the phage concentrations, resulted in no significant biofilm removal properties and the activity was not influenced by the phage contact times. The anti-biofilm activity was influenced by the age of biofilm, in this case, one day-old biofilm was more sensitive than 7 days old.

Conclusion: From the study it can be concluded, that in chicken model, the phage exhibited biofilm removal activity on Salmonella only at high concentrations, then the use of a combination of methods to control biofilm is recommended.

Full-Text [PDF 658 kb]   (174 Downloads)    
Type of Study: Research | Subject: میکروبیولوژی
Received: 2016/11/30 | Accepted: 2017/02/13 | Published: 2017/06/21

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