Volume 27, Number 11 (Monthly-Feb 2017)                   J Urmia Univ Med Sci 2017, 27(11): 1012-1024 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Soleimany L, Zare S, Hobbenaghi R, Delirezh N, Hushmandi K. COMPARISON EFFECT OF BONE MARROW DERIVED MESENCHYMAL STEM CELLS AND STIMULATED BONE MARROW MESENCHYMAL STEM CELLS WITH LPS ON HEALING OF INDUCED THIRD-DEGREE SKIN BURN IN MOUSE. J Urmia Univ Med Sci. 2017; 27 (11) :1012-1024
URL: http://umj.umsu.ac.ir/article-1-3635-en.html

Master of science Department of Biology, Faculty of Science, Urmia University, Urmia, Iran , lsoleimany@yahoo.com
Abstract:   (1784 Views)

Background & Aims: Burning provides irreparable effects on the affected patient. Several studies show that these cells may contribute to tissue regeneration whether through producing a variety of bioactive growth factors and/or by differentiation into mesoderm lineage. Several studies demonstrate that stimulated mesenchymal stem cells have more therapeutic potential than unstimulated cell in accelerate tissue wound healing. The aim of the present study was to investigate the effect of subcutaneous administration of stimulated bone marrow-derived mesenchymal stem cells with lipopolysaccharide in repairing or regeneration of skin wounds induced by third-degree burn and to compare it with unstimulated Mesenchymal stem cell in mouse model.

Materials & Methods: The third-degree skin burn was induced on male mice (N=27). After 1 hour, based on the equal physical condition mice were randomly divided into three separate groups which included control, mesenchyme and LPS groups and then respectively subcutaneously administered with phosphate buffered saline (PBS; 400 µl), mesenchymal stem cells and stimulated mesenchymal stem cells with LPS (106 cell in 400µl PBS) at the burn site. In order to check the speed of the process of 21-days wound repair, tissue section at days 7, 14, and 21 after induction of burn were prepared and stained with hematoxylin/eosin and Masson’s trichrome.

Results: Considering investigated parameters including formation of granulation tissue, angiogenesis, fibroblast proliferation, collagen deposition and the rate and quality of healing of third-degree thermal burns were significantly accelerated in mesenchyme and LPS groups when compared to the control group (P<0.05). As well as LPS group in comparison to mesenchymal group was superior in collagen deposition and rate of wound healing.

Conclusion: The results demonstrate that stimulate cells whit LPS improve BMScʼs therapeutic effect in some factors of burn wound healing.

SOURCE: URMIA MED J 2017: 27(11): 1024 ISSN: 1027-3727

Full-Text [PDF 635 kb]   (472 Downloads)    
Type of Study: Research | Subject: پوست
Received: 2016/10/10 | Accepted: 2017/01/7 | Published: 2017/02/18

Add your comments about this article : Your username or email:
Write the security code in the box

Send email to the article author


© 2015 All Rights Reserved | URMIA MEDICAL JOURNAL

Designed & Developed by : Yektaweb