Volume 27, Number 11 (Monthly-Feb 2017)                   J Urmia Univ Med Sci 2017, 27(11): 942-949 | Back to browse issues page


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Alishah M, Amini K, Zahraei Salehi T. Detection of virulence genes in Escherichia coli strains isolated from pediatric with urinary tract infection and their antibiotic resistance profile. J Urmia Univ Med Sci. 2017; 27 (11) :942-949
URL: http://umj.umsu.ac.ir/article-1-3440-en.html

Assistant Professor Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran , tsalehi@ut.ac.ir
Abstract:   (1533 Views)

Background: Urinary tract infections (UTI) are one of the most common infections worldwide. Uropathogenic Escherichia coli (UPEC), which causes urinary tract infections are the primary pathogens. The aim of this study is to identify different genes papC, papG, fimH, iutA, sfaS, ibeA and antibiotic susceptibility of E. coli strains isolated from urinary tract infection.

Methods: In this study cross-sectional, 120 samples were collected from patients with urinary tract infection referred to children medical Center in Tehran, Iran. Antibiotic susceptibility testing by disk diffusion method was performed according to CLSI guidelines. Thus,  DNA extraction was performed from all strains and  multiplex PCR was conducted for detection papC, papG, fimH, iutA, sfaS and ibeA virulance genes in all strains.

Results:  The results showed that E.coli isolates to amikacin (100%) sensitive and had resistance to ampicillin (60%). Virulence genes prevalence was fimH 85%, papC 58%, papG 47%, iutA 35%, sfaS 1% and ibeA 1%.

Conclusion: The results of this study showed that the most common gene encoding genes fimH and papC adhesion genes in E.coli was isolated from urinary tract infection Fimbriae pap. The difference between the results with other studies due to the diversity of geographic region.

Full-Text [PDF 439 kb]   (446 Downloads)    
Type of Study: Research | Subject: میکروبیولوژی
Received: 2016/06/16 | Accepted: 2016/09/26 | Published: 2017/02/15

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