Background & Aims: β-lactamase enzymes, the most important factor to antibiotic resistance among Gram-negative bacteria, is of beta-lactam family. Today, we experience increasing infection in the world and this is one of the emerging health problems in the world. CTX-M9 is a beta-lactamase resistance gene. The aim of this study was to determine the prevalence of ESBL producing Escherichia and molecular evaluation of CTX-M9 β-lactamase was amplified by PCR.
Materials & Methods: In a series 270 urine samples were collected from medical centers in the city of Sanandaj, the EMB-agar medium at 37 ° C for 24 hours and biochemical tests to confirm the 270 samples, 100 isolates of Escherichia coli was isolated. Antibiotic susceptibility using disk diffusion and ESBL enzymes were determined using the combined disk. The presence of CTX-M9 using specific primers was detected by PCR method.
Results: The drug-resistance of isolates to11 antibiotics was obtained. 27 (27%) ESBLs producing strains were detected by using combined disc method on 100 strains. PCR used for the detection of CTX-M-9 gene showed that 10 (34%) out of 27 isolates contained such gene.
Conclusion: The rate of ESBLs producing strains in the present study and various reports from other countries is highly increasing, therefore, using an appropriate treatment protocol based on the antibiogram pattern of the strains is highly recommended. The results of PCR showed a high percentage of β-lactamase resistant E. coli strains. So identification of ctx-M9 genes and Prevalence of this Genes can accelerate diagnosing and treatment of patients.
SOURCE: URMIA MED J 2016: 27(2): 105 ISSN: 1027-3727