Volume 26, Number 3 (Monthly-May 2015)                   J Urmia Univ Med Sci 2015, 26(3): 176-184 | Back to browse issues page


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Khazaie S, Jafari M, Heydari J, Salem F. INVESTIGATING RESPONSE OF SPLEEN AND ERYTHROCYTES ANTIOXIDANT DEFENSE SYSTEM ON THE EFFECTS OF N-ACETYL CYSTEINE AGAINST PARAOXON TOXICITY IN RAT. J Urmia Univ Med Sci. 2015; 26 (3) :176-184
URL: http://umj.umsu.ac.ir/article-1-2802-en.html

Professor of Biochemistry Department of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran , m.jafari145@gmail.com
Abstract:   (2943 Views)

 

  Background & Aims : Paraoxon (POX) as an organophosphate pesticide is the active form of parathion that is widely used in agriculture. Antioxidants can protect cells from oxidative stress. The aim of this study was to investigate the effect of NAC as a n antioxidant against POX- induced oxidative stress in rat spleen and erythrocytes.

  Material & Methods : In the present experimental study, male Wistar rats were randomly divided into four groups including: control group (corn oil as POX solvent), POX group (0.7 mg/kg), NAC group (160 mg/kg), and NAC+POX all of which were given intraperitoneally. Then, 24 hours after injection, the animals were anesthetized, spleen tissues were quickly removed and blood was also collected by cardiac puncture and erythrocytes were obtained. Then, superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) activities, as well as glutathione (GSH) and malondialdehyde (MDA) levels were determined by biochemical methods.

  Results: POX increased SOD and GST activities and decreased GSH content in rat spleen and erythrocytes. Also, POX increased CAT activity and MDA level in erythrocytes and decreased CAT activity in spleen. Administration of NAC inhibited the changes in these parameters.

  Conclusion: POX with free radical production and depleted GSH content leads to oxidative stress induction in spleen and erythrocytes. Administration of NAC as antioxidant decreases POX-induced oxidative stress by scaveng ing free radicals and GSH synthesis , but it does not protect completely.

 

  SOURCE: URMIA MED J 2015: 26(3): 184 ISSN: 1027-3727

Full-Text [PDF 276 kb]   (603 Downloads)    
Type of Study: Research | Subject: آناتومی
Received: 2015/05/27 | Accepted: 2015/05/27 | Published: 2015/05/27

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