Volume 25, Issue 5 (Monthly-July 2014)                   J Urmia Univ Med Sci 2014, 25(5): 453-462 | Back to browse issues page


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Khosravi M, Mossavi N S, Parizadeh S M R, Aghagolzade Haji H. INVESTIGATING PLATELET RICH PLASMA EFFECTS ON ADIPOSE-DERIVED STROMAL CELLSPROLIFERATION AND DIFFERENTIATION TO OSTEOBLAST. J Urmia Univ Med Sci. 2014; 25 (5) :453-462
URL: http://umj.umsu.ac.ir/article-1-2331-en.html

Department of Clinical Biochemistry, Mashhad University of Medical Sciences , khosravi.dara681@gmail.com
Abstract:   (3576 Views)

 

  Background & Aims: The cultured mesenchymal stem cells (MSCs) have been applied in many clinical trials and there are some concerns about the culture conditions. One of them is related to the use of fetal bovine serum (FBS). FBS is a xoengenic supplement withadverse effects which is used widely in culture system and many attempts have been done to eliminate it. Platelet rich plasma (PRP) is a candidate in the place of FBS.

  Materials & Methods: Adipose-derived stromal cells (ADSCs) were isolated from liposuction tissues and cultured in αMEM with 10 % FBS. Cells of the third passage were used for the in vitro experiments and characterized by flowcytometric analysis . PRP was obtained by two-step centrifugation and PRP sample were activated by freezing method. Resazurinand mineralization assays were used to determine the effects of PRP on cell proliferation and osteogenic differentiation.

  Results: Treatment with PRP resulted in a statistically significant increase cell proliferation and mineralization in comparison to negative control (P<0.001) and that of the 15% PRP group significantly was higher than that of 10 % PRP group (P<0.05) . FBS showed the osteogenic differentiation of ADSCs better than hPRP.

  Conclusions: These findings indicate that PRP improves the proliferation and osteogenic differentiation of ADSCs. Obviously, the biological effects of PRP were dose-dependent and can be a useful solution for clinical applications in the place of FBS. However, for the clinical application of PRP, more research is needed such as in vivo transplantation.

 

  SOURCE: URMIA MED J 2014: 25(5): 462 ISSN: 1027-3727

 

  Address: Department of Clinical Biochemistry, Mashhad University of Medical Sciences, Mashhad, Iran, Tel: +98 9124511983

  Email: khosravi.dara681@gmail.com

 

  SOURCE: URMIA MED J 2014: 25(5): 462 ISSN: 1027-3727

Full-Text [PDF 506 kb]   (901 Downloads)    
Type of Study: Research | Subject: آناتومی
Received: 2014/07/28 | Accepted: 2014/07/28 | Published: 2014/07/28

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