Volume 24, Number 10 (Monthly_Dec 2013)                   J Urmia Univ Med Sci 2013, 24(10): 753-762 | Back to browse issues page


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Niknejad H, Yazdanpanah G, Deihim T. THE EFFECTS OF CRYOPRESERVATION AND LYOPHILIZATION ON ENDOTHELIAL CELLS ADHESION TO HUMAN AMNIOTIC MEMBRANE. J Urmia Univ Med Sci. 2013; 24 (10) :753-762
URL: http://umj.umsu.ac.ir/article-1-1981-en.html

Assistant Professor of pharmachology Nanomedicine and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences , niknejad@sbmu.ac.ir
Abstract:   (5042 Views)
Background & Aims: Human amniotic membrane has some specific properties making it an appropriate biomaterial for using in vascular tissue engineering. In this study, amniotic membrane was preserved with different methods. Effects of preservation on amniotic extracellular matrix and adhesion of cultured endothelial cells to membrane were compared with fresh samples of amniotic membrane. Materials & Methods: Human amniotic membrane was preserved with cryopreservation (-80 ̊C for 12 month) or lyophilization methods. Extracellular matrix components were assayed with immunohistochemistry method. The adhesion of cultured endothelial cells was studied with MTT assay. Results between groups were compared with ANOVA (Post-test Tukey). Results: Results demonstrated that extracellular matrix components were same in cryopreserved samples in comparison to fresh ones but there are some differences in lyophilized samples. Adhesion of endothelial cells to lyophilized samples was significantly more than cryopreserved or fresh samples (P Value < 0.05). Conclusion: Both cryopreservation and lyophilization affect extracellular matrix of human amniotic membrane which can determine the rate of the adhesion of endothelial cells to amniotic membrane. Lyophilized amniotic membrane is a better choice for culture of endothelial cells in vascular tissue engineering. SOURCE: URMIA MED J 2013: 24(10): 762 ISSN: 1027-3727
Full-Text [PDF 547 kb]   (952 Downloads)    
Type of Study: Research | Subject: آناتومی
Received: 2013/12/25 | Accepted: 2013/12/25 | Published: 2013/12/25

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