Volume 23, Number 7 (3-2013)                   J Urmia Univ Med Sci 2013, 23(7): 792-798 | Back to browse issues page


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Yakhchali M, Mardani K. A STUDY ON ECHINOCOCCUS GRANULOSUS STRAINS USING NUCLEOTIDE SEQUENCE OF CO-1 GENE BY PCR-RFLP TWCHNIQUE IN WEST AZARBAIJAN PROVINCE, IRAN. J Urmia Univ Med Sci. 2013; 23 (7) :792-798
URL: http://umj.umsu.ac.ir/article-1-1600-en.html

Associate Professor , Parasitology division, Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , m.yakhchali@urmia.ac.ir
Abstract:   (5972 Views)

  Received: 3 Oct, 2012 Accepted: 21 Des, 2012

  

  Background & Aims : Identification of genotypic variation of Echinococcus granulosus strains is important for epidemiology and control of the larval stage of this parasite. The present study was undertaken in order to identify E. granulosus strains in West Azerbaijan province , Iran .

  Materials & Methods : Organs infected with hydatid cysts from slaughtered ruminants including 115 liver and 155 lungs from sheep, 59 liver and 126 lungs from goats, 119 liver and 78 lungs from cattle and 129 livers from water buffalos were obtained from slaughterhouses of Khoy (north), Urmia (central), and Mahabad (south). After disinfection of each cyst surface, hydatid cyst fluid was aspirated and centrifuged for collecting protoscolices . A number of 114 fertile hydatid cysts were used for DNA extraction. To determine genotypic variation of E. granulosus, a fragment of 1213 bp of mitochondrial cytochrom oxidase subunit 1 (co-1) gene was amplified (PCR). Obtained PCR products were subjected to restriction fragment length polymorphism (RFLP) analysis using HaeIII endonuclease .

  Results : A fragment of 1213 bp in size of co-1 gene was amplified successfully form all hydatid cysts of examined ruminants. Amplified PCR products from hydatid cysts of cattle, sheep, and goats generated similar RFLP patterns, but different RFLP pattern for hydatid cysts from buffaloes.

  Conclusion : The RFLP patterns of the amplified fragment of the co-1 gene of E. granulosus indicated the circulation of two different E. granolusus strains of sheep (G1) and water buffalo (G3) in northwestern of Iran .

  

  SOURCE: URMIA MED J 2012: 23(7): 829 ISSN: 1027-3727

Full-Text [PDF 164 kb]   (890 Downloads)    
Type of Study: Research | Subject: آناتومی
Received: 2013/03/18 | Accepted: 2013/03/18 | Published: 2013/03/18

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