Volume 27, Issue 12 (Monthly-Mar 2017)                   J Urmia Univ Med Sci 2017, 27(12): 1074-1081 | Back to browse issues page



DOI: 10.18869/acadpub.umj.27.12.1074

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naderi N, seyed majidi M, sarrafan N, salehinejad J, gholinia H, abbaszadeh bidokhti H. Evaluation of proliferation activity of oral lichen planus and oral lichenoid reactions using quantitative and qualitative analysis of AgNORs (Argyrophilic Nucleolar Organizer Regions). J Urmia Univ Med Sci. 2017; 27 (12) :1074-1081
URL: http://umj.umsu.ac.ir/article-1-3483-en.html

assistant professor Assistant Professor, cellular and molecular biology research center, Department of Oral and Maxillofacial Pathology, Dental School,Babol University of Medical Sciences, Babol, Iran (Corresponding Author) , hamidabbaszade@yahoo.com
Abstract:   (1384 Views)

Bachground & Aims: Oral lichenoid reactions (OLR) and oral lichen planus (OLP) are clinically and histopathologically similar but their therapeutic approach are different. Due to the presence of dysplasia in oral lichen planus which effects prognosis, the aim of study was the evaluation of proliferation activity of OLPs and OLRs and their subtypes. AgNOR technique that is inexpensive and easy to perform was used to differentiate OLPs and OLRs and their subtypes.

Materials & Methods: in this cross sectional , retrospective study, samples consisted of 45 OLPs, 30 OLRs 15 normal oral mucosa (N) from healthy persons (as control group). The samples were stained by AgNOR technique. AgNOR dots were counted on 100 cells from basal and para basal layer . The mean AgNOR dost per nucleus were calculated. Proliferation index (number of cells with AgNOR dots≥ 5) was also determined. Variation in size of AgNOR dots was categorized into 2 groups.

Result: There were significant differences between OLP(and its subtypes) and N and also between OLR(and erosive type) and N . Such a difference was also between reticular OLP and reticular OLR.with regard to AgNOR count.

Conclusion: Since reticular OLP had significantly higher AgNOR count than reticular OLR in our study, we can suggest the use of AgNOR technique for differentiation of reticular type of OLP from OLR on challenging histopathologic cases.

Full-Text [PDF 337 kb]   (575 Downloads)    
Type of Study: Research | Subject: Oral Pathology
Received: 2016/07/8 | Accepted: 2016/09/11 | Published: 2017/03/12

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